Cui Qing-Zhi, Tian Yan-Jun, Wen Hai-Xia, Zhang Guo-Dong, Han Rui-Na, Zhang Guang-Mei, Li Guo-Yi
Department of Physiology, Harbin Medical University, Harbin 150081, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2010 Aug;26(3):318-21.
To investigate the effects of Yikunning (compound of Chinese traditional Medicine, YKN) on the apoptotic rate and expression of caspase-3 in rat ovaries during perimenopausal period.
Thirty female Wistar rats during perimenopausal period were selected by unforced aging. Then the rats were divided into 3 groups randomly: YKN group, livial control group and aged control group. Ten young female rats were selected as young control group. Intragastric administrations were conducted for 4 weeks once daily continuously. The apoptotic rate in rat ovaries were detected by TUNEL. The expression of caspase-3 mRNA and protein in rat ovaries were detected by RT-PCR and Western blot, respectively.
The apoptotic rate in rat ovaries in YKN group was lower than that in aged control group, which showed difference between them (P < 0.01). The levels of caspase-3 mRNA and protein in rat ovaries in YKN group were lower than those in aged control group, which showed differences among them (P < 0.01).
YKN can decrease the apoptotic rate and down-regulate the expression of caspase-3 mRNA and protein in rat ovaries of during perimenopausal period. It may be one of the molecular mechanisms of YKN postponed the ovarian failure and cured perimenopausal syndrome.
探讨益坤宁（一种中药复方，YKN）对围绝经期大鼠卵巢细胞凋亡率及半胱天冬酶 - 3（caspase - 3）表达的影响。
采用自然衰老法选取30只围绝经期雌性Wistar大鼠，随机分为3组：益坤宁组、莉芙敏对照组和老龄对照组。选取10只青年雌性大鼠作为青年对照组。连续4周每日灌胃给药1次。采用TUNEL法检测大鼠卵巢细胞凋亡率，分别采用RT - PCR法和Western blot法检测大鼠卵巢中caspase - 3 mRNA和蛋白的表达。
益坤宁组大鼠卵巢细胞凋亡率低于老龄对照组，差异有统计学意义（P < 0.01）。益坤宁组大鼠卵巢中caspase - 3 mRNA和蛋白水平低于老龄对照组，差异有统计学意义（P < 0.01）。
益坤宁可降低围绝经期大鼠卵巢细胞凋亡率，下调caspase - 3 mRNA和蛋白的表达。这可能是益坤宁延缓卵巢衰老、治疗围绝经期综合征的分子机制之一。
