Wen Hai-Xia, Xiao Xiao-Hui, Zhao Wei, Liu Guo-Yi, Song Hong-Zhe, Ni Jiang
Department of Physiology, Harbin Medical University, Harbin 150086, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007 Feb;23(1):117-20.
To investigate the effects of soy isoflavones (SI) on the expression of Bax mRNA and Ca(2+) -ATPase activity in ovaries of perimenopause rats.
The animal model of perimenopause rats was established by unforced aging. 12 month-old presenilins female Wistar rats were administered by intragastric (ig) with low (500 mg/kg), middle (158 mg/kg) and high (500 mg/kg) does of SI for 8 weeks. The expression of Bax mRNA in ovaries were detected by RT-PCR. Ca(2+) -ATPase activity in ovaries and MDA content and SOD activity in serum were detected by chemi-chromatometry.
Intervention of SI could significantly decrease the expression of Bax mRNA in ovaries and MDA content in serum, increase Ca(2+) -ATPase activity in ovaries and SOD activity in serum of presenilins rats (P < 0.05 or P < 0.01).
Soy isoflavones could down-regulate the expression of Bax mRNA and increase Ca(2+) -ATPase activity in aged ovaries. It is probably one of the mechanisms to improve the function of aged ovaries in perimenopause rats.
探讨大豆异黄酮（SI）对围绝经期大鼠卵巢中Bax mRNA表达及Ca(2+) -ATP酶活性的影响。
采用自然衰老法建立围绝经期大鼠动物模型。对12月龄的早老素雌性Wistar大鼠进行灌胃给予低剂量（500毫克/千克）、中剂量（158毫克/千克）和高剂量（500毫克/千克）的SI，持续8周。采用逆转录聚合酶链反应（RT-PCR）检测卵巢中Bax mRNA的表达。采用化学比色法检测卵巢中Ca(2+) -ATP酶活性以及血清中丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性。
SI干预可显著降低早老素大鼠卵巢中Bax mRNA的表达及血清中MDA含量，增加卵巢中Ca(2+) -ATP酶活性及血清中SOD活性（P < 0.05或P < 0.01）。
大豆异黄酮可下调衰老卵巢中Bax mRNA的表达并增加Ca(2+) -ATP酶活性。这可能是改善围绝经期大鼠衰老卵巢功能的机制之一。
