MSPCA Angell Animal Medical Center, 350 S. Huntington Ave., Boston, MA 02130, USA.
J Anim Physiol Anim Nutr (Berl). 2011 Feb;95(1):90-7. doi: 10.1111/j.1439-0396.2010.01016.x. Epub 2010 Oct 29.
This study evaluated four over the counter venison dry dog foods available from one on-line retail vendor for potential contamination with common known food allergens: soy, poultry or beef. An amplified, double sandwich type enzyme linked immunosorbent assay (ELISA) test of soy, poultry and beef proteins were performed by an independent accredited food laboratory. The ELISA test for poultry protein was found to be unreliable when testing in dry dog foods because false negatives occurred. ELISA testing of control diets for both soy and beef proteins performed as expected and could be useful in antigen testing in dry dog foods. Three of the four over the counter (OTC) venison canine dry foods with no soy products named in the ingredient list were ELISA positive for soy; additionally one OTC diet tested positive for beef protein with no beef products listed as an ingredient list. One OTC venison diet was not found to be positive for soy, poultry or beef proteins. However, none of the four OTC venison diets could be considered suitable for a diagnostic elimination trial as they all contained common pet food proteins, some of which were readily identifiable on the label and some that were only detected by ELISA. Therefore, if the four OTC venison products selected in this study are representative of OTC products in general, then the use of OTC venison dry dog foods should not be used during elimination trials in suspected food allergy patients.
本研究评估了从一个在线零售商处购买的四种市售鹿肉干狗粮，以评估其是否存在常见的已知食物过敏原（大豆、禽肉或牛肉）污染的可能性。采用独立认可的食品实验室，对大豆、禽肉和牛肉蛋白进行了放大的双夹心酶联免疫吸附试验（ELISA）检测。在干狗粮中进行禽肉蛋白 ELISA 检测时，结果不可靠，因为出现了假阴性。大豆和牛肉蛋白的对照饮食的 ELISA 检测结果符合预期，并且可能对干狗粮中的抗原检测有用。四种市售（OTC）鹿肉犬干粮中，有三种未在成分表中列出大豆制品，但却被 ELISA 检测出大豆阳性；此外，有一种 OTC 饮食被检测出含有牛肉蛋白，但成分表中并未列出牛肉制品。有一种 OTC 鹿肉饮食未被检测出大豆、禽肉或牛肉蛋白阳性。然而，这四种 OTC 鹿肉饮食都不能被认为适合用于诊断性消除试验，因为它们都含有常见的宠物食品蛋白，其中一些在标签上可以轻易识别，而有些则只能通过 ELISA 检测到。因此，如果本研究中选择的四种 OTC 鹿肉产品能够代表一般的 OTC 产品，那么在疑似食物过敏患者的消除试验中，不应使用市售的 OTC 鹿肉干狗粮。
