Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Clin Exp Allergy. 2011 Feb;41(2):171-8. doi: 10.1111/j.1365-2222.2010.03584.x. Epub 2010 Oct 7.
Fungi and/or Staphylococcus aureus enterotoxins (SEs) may participate in the pathogenesis of eosinophilic inflammation in cases of chronic rhinosinusitis with nasal polyps (CRSwNP). Objective We sought to determine the effects of fungal antigens on eosinophilia-associated cellular responses in nasal polyps.
Dispersed nasal polyp cells (DNPCs) were prepared from 13 patients with CRSwNP. DNPCs were cultured with fungal extracts (Aspergillus, Alternaria and Candida) or SEB for 72 h, after which the levels of IL-5, IL-13 and RANTES were measured within the supernatant. Responses to β-d-glucan, mannan and chitin were also examined.
38.5%, 69.2% and 30.8% of DNPCs produced IL-5, IL-13 and RANTES, respectively, in response to 200 μg/mL of Aspergillus. 53.8%, 53.8% and 7.7% of DNPCs produced IL-5, IL-13 and RANTES, respectively, in response to 200 μg/mL of Alternaria. 53.8%, 38.5% and 15.4% of DNPCs produced IL-5, IL-13 and RANTES, respectively, in response to 200 μg/mL of Candida. All DNPCs produced these cytokines in response to 0.1 μg/mL of SEB. SEB induced significantly greater cytokine levels than the fungal extracts. No correlation between cytokine production following exposure to each of the fungal extracts or SEB and various clinical features, including nasal polyp eosinophilia and radiological severity of sinusitis was observed. Neither sensitization to fungus nor comorbidity with bronchial asthma was correlated with the fungal extract-induced cytokine production by DNPCs. β-d-glucan, mannan and chitin did not induce significant cytokine production.
These results suggest that, although DNPCs produce IL-5, IL-13 and RANTES in response to fungal extracts, fungal antigens including major carbohydrates are less capable of inducing eosinophilia-associated cellular responses in nasal polyps than SEB.
真菌和/或金黄色葡萄球菌肠毒素（SEs）可能参与慢性鼻-鼻窦炎伴鼻息肉（CRSwNP）中嗜酸性粒细胞炎症的发病机制。目的 我们旨在确定真菌抗原对鼻息肉中嗜酸性粒细胞相关细胞反应的影响。
从 13 例 CRSwNP 患者中制备分散的鼻息肉细胞（DNPCs）。DNPCs 用真菌提取物（曲霉、交链孢霉和念珠菌）或 SEB 培养 72 h，然后测量上清液中 IL-5、IL-13 和 RANTES 的水平。还检查了对β-d-葡聚糖、甘露聚糖和几丁质的反应。
DNPC 对 200 μg/mL 曲霉的反应分别产生 IL-5、IL-13 和 RANTES 的比例为 38.5%、69.2%和 30.8%。DNPC 对 200 μg/mL 交链孢霉的反应分别产生 IL-5、IL-13 和 RANTES 的比例为 53.8%、53.8%和 7.7%。DNPC 对 200 μg/mL 念珠菌的反应分别产生 IL-5、IL-13 和 RANTES 的比例为 53.8%、38.5%和 15.4%。所有 DNPC 对 0.1 μg/mL SEB 均产生这些细胞因子。SEB 诱导的细胞因子水平明显高于真菌提取物。未观察到暴露于每种真菌提取物或 SEB 后细胞因子的产生与各种临床特征（包括鼻息肉嗜酸性粒细胞和鼻窦炎的放射学严重程度）之间存在相关性。真菌致敏或与支气管哮喘共存均与 DNPC 对真菌提取物诱导的细胞因子产生无关。β-d-葡聚糖、甘露聚糖和几丁质没有诱导显著的细胞因子产生。
这些结果表明，尽管 DNPC 对真菌提取物产生 IL-5、IL-13 和 RANTES，但真菌抗原（包括主要碳水化合物）在鼻息肉中诱导嗜酸性粒细胞相关细胞反应的能力低于 SEB。
