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氯离子细胞内通道1在内皮细胞生长和迁移中发挥作用。

Chloride intracellular channel 1 functions in endothelial cell growth and migration.

作者信息

Tung Jennifer J, Kitajewski Jan

机构信息

Department of Obstetrics/Gynecology, Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, 1130 St, Nicholas Ave, 926, New York, NY 10032, USA.

出版信息

J Angiogenes Res. 2010 Nov 1;2:23. doi: 10.1186/2040-2384-2-23.

DOI:10.1186/2040-2384-2-23
PMID:21040583
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2993651/
Abstract

BACKGROUND

Little is known about the role of CLIC1 in endothelium. These studies investigate CLIC1 as a regulator of angiogenesis by in vitro techniques that mimic individual steps in the angiogenic process.

METHODS

Using shRNA against clic1, we determined the role of CLIC1 in primary human endothelial cell behavior.

RESULTS

Here, we report that reduced CLIC1 expression caused a reduction in endothelial migration, cell growth, branching morphogenesis, capillary-like network formation, and capillary-like sprouting. FACS analysis showed that CLIC1 plays a role in regulating the cell surface expression of various integrins that function in angiogenesis including β1 and α3 subunits, as well as αVβ3 and αVβ5.

CONCLUSIONS

Together, these results indicate that CLIC1 is required for multiple steps of in vitro angiogenesis and plays a role in regulating integrin cell surface expression.

摘要

背景

关于CLIC1在内皮细胞中的作用知之甚少。这些研究通过模拟血管生成过程中各个步骤的体外技术，将CLIC1作为血管生成的调节因子进行研究。

方法

使用针对clic1的短发夹RNA（shRNA），我们确定了CLIC1在原代人内皮细胞行为中的作用。

结果

在此，我们报告CLIC1表达降低导致内皮细胞迁移、细胞生长、分支形态发生、毛细血管样网络形成和毛细血管样芽生减少。流式细胞术分析表明，CLIC1在调节血管生成中起作用的各种整合素的细胞表面表达中发挥作用，包括β1和α3亚基，以及αVβ3和αVβ5。

结论

总之，这些结果表明CLIC1是体外血管生成多个步骤所必需的，并在调节整合素细胞表面表达中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2332/2993651/ebfd24d6d400/2040-2384-2-23-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2332/2993651/de145d5eb810/2040-2384-2-23-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2332/2993651/98d733fbb084/2040-2384-2-23-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2332/2993651/2ce201ae3e31/2040-2384-2-23-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2332/2993651/acc3475aa8b8/2040-2384-2-23-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2332/2993651/fa0577853ace/2040-2384-2-23-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2332/2993651/ebfd24d6d400/2040-2384-2-23-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2332/2993651/de145d5eb810/2040-2384-2-23-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2332/2993651/98d733fbb084/2040-2384-2-23-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2332/2993651/2ce201ae3e31/2040-2384-2-23-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2332/2993651/acc3475aa8b8/2040-2384-2-23-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2332/2993651/fa0577853ace/2040-2384-2-23-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2332/2993651/ebfd24d6d400/2040-2384-2-23-6.jpg

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1
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2
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Am J Physiol Renal Physiol. 2010 Jun;298(6):F1492-503. doi: 10.1152/ajprenal.00030.2010. Epub 2010 Mar 24.
3
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Front Oncol. 2024 Apr 30;14:1375190. doi: 10.3389/fonc.2024.1375190. eCollection 2024.
4
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Am J Cancer Res. 2023 Aug 15;13(8):3300-3314. eCollection 2023.
5
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7
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Biotechniques. 2009 Apr;46(4):265-74. doi: 10.2144/000113083.
8
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9
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