Department of Laboratory Medicine, School of Medicine, Keio University, Tokyo, Japan.
Biochem Biophys Res Commun. 2010 Nov 26;402(4):796-800. doi: 10.1016/j.bbrc.2010.10.120. Epub 2010 Oct 30.
Platelets are produced from megakaryocytes (MKs), although the pathway leading from stem cells to MK lineages are not yet fully understood. Recently, we reported to obtain abundant MKs and platelets from human subcutaneous adipose tissues. Adipose tissues contain various cell types, most of which are lineage cells from mesenchymal or adipocyte-derived stem cells, distinct from hematopoietic cells. To identify the cells responsible for the differentiation MK lineages in adipose tissues, this study examined whether the preadipocyte cell line 3T3-L1 and fibroblast cell line 3T3 differentiated into MK lineages in vitro. Cells were cultured in megakaryocyte lineage induction medium. By day 4, most of 3T3 cell-derived cells leaded to cell death. In contrast, 3T3-L1-derived cells on days 8 showed to have typical characterizations of MK lineages in analyses for specific marker, DNA ploidy, transmission electro micrograph. 3T3-L1-derived platelet-sized cells on day 12 could be stimulated by ADP and PAR4-activating peptide. This study clearly shows in vitro differentiation from 3T3-L1 cells, not from 3T3 cells, into MK lineages.
血小板来源于巨核细胞（MKs），尽管从干细胞到 MK 谱系的途径尚未完全清楚。最近，我们报道了从人皮下脂肪组织中获得丰富的 MK 和血小板。脂肪组织包含各种细胞类型，其中大多数是间充质或脂肪干细胞来源的谱系细胞，与造血细胞不同。为了鉴定脂肪组织中分化为 MK 谱系的细胞，本研究检查了前脂肪细胞系 3T3-L1 和成纤维细胞系 3T3 是否能在体外分化为 MK 谱系。细胞在巨核细胞谱系诱导培养基中培养。到第 4 天，大多数 3T3 细胞来源的细胞导致细胞死亡。相比之下，在第 8 天，3T3-L1 衍生的细胞在分析特定标志物、DNA 倍性、透射电镜时表现出典型的 MK 谱系特征。在第 12 天，3T3-L1 衍生的血小板大小的细胞可被 ADP 和 PAR4 激活肽刺激。本研究清楚地表明，3T3-L1 细胞而非 3T3 细胞在体外分化为 MK 谱系。
