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In vitro generation of platelets through direct conversion: first report in My Knowledge (iMK).通过直接转化在体外生成血小板：My Knowledge(iMK)中的首次报告。
Cell Res. 2013 Feb;23(2):176-8. doi: 10.1038/cr.2012.142. Epub 2012 Oct 9.
2
Induction of functional platelets from mouse and human fibroblasts by p45NF-E2/Maf.由 p45NF-E2/Maf 诱导的来自于小鼠和人类成纤维细胞的功能性血小板。
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[Induction of megakaryocytes and platelet production from mouse and human fibroblasts by p45 NF-E2/Maf].[p45 NF-E2/Maf诱导小鼠和人成纤维细胞生成巨核细胞和血小板]
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Generation of functional platelets from canine induced pluripotent stem cells.从犬诱导多能干细胞生成功能性血小板。
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Transient activation of c-MYC expression is critical for efficient platelet generation from human induced pluripotent stem cells.瞬时激活 c-MYC 表达对于从人诱导多能干细胞中有效生成血小板至关重要。
J Exp Med. 2010 Dec 20;207(13):2817-30. doi: 10.1084/jem.20100844. Epub 2010 Nov 22.
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[Direct reprogramming of fibroblasts and preadipocytes into functional megakaryocytes].[成纤维细胞和前脂肪细胞直接重编程为功能性巨核细胞]
Rinsho Ketsueki. 2014 Apr;55(4):387-95.
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Expandable megakaryocyte cell lines enable clinically applicable generation of platelets from human induced pluripotent stem cells.可膨胀巨核细胞系使人类诱导多能干细胞生成血小板的临床应用成为可能。
Cell Stem Cell. 2014 Apr 3;14(4):535-48. doi: 10.1016/j.stem.2014.01.011. Epub 2014 Feb 13.
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Generation and characterization of HLA-universal platelets derived from induced pluripotent stem cells.诱导多能干细胞来源的 HLA 通用性血小板的生成和鉴定。
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NF-E2 p45 is important for establishing normal function of platelets.NF-E2 p45 对于血小板的正常功能很重要。
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Pluripotent stem cells reveal the developmental biology of human megakaryocytes and provide a source of platelets for clinical application.多能干细胞揭示了人类巨核细胞的发育生物学，并为临床应用提供了血小板的来源。
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Hematopoietic stem cells and HSCs-derived from pluripotent stem cells: a hopeful outlook in cell-based therapeutics.造血干细胞以及源自多能干细胞的造血干细胞：基于细胞的治疗的一个充满希望的前景。
Mol Biol Rep. 2025 Jun 30;52(1):657. doi: 10.1007/s11033-025-10730-w.
2
Generation of HLA Universal Megakaryocytes and Platelets by Genetic Engineering.通过基因工程生成 HLA 通用巨核细胞和血小板。
Front Immunol. 2021 Oct 27;12:768458. doi: 10.3389/fimmu.2021.768458. eCollection 2021.
3
The Opportunities and Challenges regarding Induced Platelets from Human Pluripotent Stem Cells.人多能干细胞诱导血小板面临的机遇与挑战
Stem Cells Int. 2021 May 1;2021:5588165. doi: 10.1155/2021/5588165. eCollection 2021.
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Inhibition of Tropomyosin Receptor Kinase A Signaling Negatively Regulates Megakaryopoiesis and induces Thrombopoiesis.抑制原肌球蛋白受体激酶 A 信号通路可负向调节巨核细胞生成并诱导血小板生成。
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Platelet generation in vivo and in vitro.体内和体外血小板生成
Springerplus. 2016 Jun 21;5(1):787. doi: 10.1186/s40064-016-2384-1. eCollection 2016.
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Platelet bioreactor-on-a-chip.芯片上的血小板生物反应器
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Manufacturing blood ex vivo: a futuristic approach to deal with the supply and safety concerns.体外制造血液：应对供应和安全问题的未来主义方法。
Front Cell Dev Biol. 2014 Jun 11;2:26. doi: 10.3389/fcell.2014.00026. eCollection 2014.

本文引用的文献

1
Therapeutic platelet transfusion versus routine prophylactic transfusion in patients with haematological malignancies: an open-label, multicentre, randomised study.治疗性血小板输注与血液病患者常规预防性血小板输注的比较：一项开放标签、多中心、随机研究。
Lancet. 2012 Oct 13;380(9850):1309-16. doi: 10.1016/S0140-6736(12)60689-8. Epub 2012 Aug 8.
2
Stem-cell pioneer banks on future therapies.干细胞先驱寄望于未来疗法。
Nature. 2012 Aug 9;488(7410):139. doi: 10.1038/488139a.
3
Generating an iPSC bank for HLA-matched tissue transplantation based on known donor and recipient HLA types.基于已知供者和受者 HLA 类型，生成与 HLA 匹配的组织移植用 iPSC 库。
Cell Stem Cell. 2012 Aug 3;11(2):147-52. doi: 10.1016/j.stem.2012.07.014.
4
Induction of functional platelets from mouse and human fibroblasts by p45NF-E2/Maf.由 p45NF-E2/Maf 诱导的来自于小鼠和人类成纤维细胞的功能性血小板。
Blood. 2012 Nov 1;120(18):3812-21. doi: 10.1182/blood-2012-02-413617. Epub 2012 Jul 31.
5
Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells.人诱导多能干细胞肝向分化的供体依赖性差异。
Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12538-43. doi: 10.1073/pnas.1209979109. Epub 2012 Jul 16.
6
A more efficient method to generate integration-free human iPS cells.一种更有效的生成无整合的人诱导多能干细胞的方法。
Nat Methods. 2011 May;8(5):409-12. doi: 10.1038/nmeth.1591. Epub 2011 Apr 3.
7
Platelets generated from human embryonic stem cells are functional in vitro and in the microcirculation of living mice.人胚胎干细胞生成的血小板在体外和活体小鼠的微循环中具有功能。
Cell Res. 2011 Mar;21(3):530-45. doi: 10.1038/cr.2011.8. Epub 2011 Jan 11.
8
Transient activation of c-MYC expression is critical for efficient platelet generation from human induced pluripotent stem cells.瞬时激活 c-MYC 表达对于从人诱导多能干细胞中有效生成血小板至关重要。
J Exp Med. 2010 Dec 20;207(13):2817-30. doi: 10.1084/jem.20100844. Epub 2010 Nov 22.
9
Generation of megakaryocytes and platelets from preadipocyte cell line 3T3-L1, but not the parent cell line 3T3, in vitro.体外诱导前脂肪细胞系 3T3-L1 而非亲本细胞系 3T3 生成巨核细胞和血小板。
Biochem Biophys Res Commun. 2010 Nov 26;402(4):796-800. doi: 10.1016/j.bbrc.2010.10.120. Epub 2010 Oct 30.
10
Epigenetic memory in induced pluripotent stem cells.诱导多能干细胞中的表观遗传记忆。
Nature. 2010 Sep 16;467(7313):285-90. doi: 10.1038/nature09342.

通过直接转化在体外生成血小板：My Knowledge(iMK)中的首次报告。

In vitro generation of platelets through direct conversion: first report in My Knowledge (iMK).

机构信息

Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.

出版信息

Cell Res. 2013 Feb;23(2):176-8. doi: 10.1038/cr.2012.142. Epub 2012 Oct 9.

DOI:10.1038/cr.2012.142
PMID:23044804
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3567822/
Abstract

Blood transfusion medicine requires a constant supply of platelets, which is now totally donor dependent. Recent advances in the generation of platelets in vitro through megakaryocytes (MKs) may provide protocols not only via pluripotent stem cells, including induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs), but also via induced MKs (iMKs). For the first time, mouse and human fibroblasts are successfully transdifferentiated into iMKs by the introduction of three factors, p45NF-E2, Maf G, and Maf K.

摘要

输血医学需要不断供应血小板，而目前完全依赖于供体。通过巨核细胞 (MKs) 在体外生成血小板的最新进展，不仅可以通过多能干细胞（包括诱导多能干细胞（iPSCs）和胚胎干细胞（ESCs）），还可以通过诱导的 MKs (iMKs) 提供方案。首次通过引入三个因子 p45NF-E2、Maf G 和 Maf K，成功地将小鼠和人成纤维细胞转分化为 iMKs。