Institute of Chemical Process Fundamentals, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Biophys Chem. 2010 Dec;153(1):54-60. doi: 10.1016/j.bpc.2010.10.004. Epub 2010 Oct 13.
The immobilization of cells or enzymes is a promising tool for the development of biosensors, yet the interactions between the fixative materials and the cells are not fully understood, especially with respect to their impact on both cell metabolism and cell-to-cell signaling. We show that the spatiotemporal dynamics of waves of metabolic synchronization of yeast cells provides a new criterion to distinguish the effect of different gels on the cellular metabolism, which otherwise could not be detected. Cells from the yeast Saccharomyces carlsbergensis were immobilized into agarose gel, silica gel (TMOS), or a mixture of TMOS and alginate. We compared these immobilized cells with respect to their ability to generate temporal, intracellular oscillations in glycolysis as well as propagating, extracellular synchronization waves. While the temporal dynamics, as measured by the period and the number of oscillatory cycles, was similar for all three immobilized cell populations, significant differences have been observed with respect to the shape of the waves, wave propagation direction and velocity in the three gel matrices used.
细胞或酶的固定化是生物传感器发展的一种很有前途的工具，但固定材料与细胞之间的相互作用还不完全清楚，特别是它们对细胞代谢和细胞间信号传递的影响。我们表明，酵母细胞代谢同步波的时空动力学为区分不同凝胶对细胞代谢的影响提供了一个新的标准，否则这些影响是无法检测到的。将酿酒酵母（Saccharomyces carlsbergensis）的细胞固定在琼脂糖凝胶、硅胶（TMOS）或 TMOS 和藻酸盐的混合物中。我们比较了这三种固定化细胞在产生糖酵解的时间、细胞内振荡以及传播的细胞外同步波的能力。虽然所有三种固定化细胞群体的时间动力学（通过周期和振荡循环数来衡量）相似，但在三种凝胶基质中观察到波的形状、波传播方向和速度存在显著差异。
