miR-206 和 -486 通过下调 Pax7 诱导成肌细胞分化。
miR-206 and -486 induce myoblast differentiation by downregulating Pax7.
机构信息
Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Jordan Hall 1232, Charlottesville, VA 22908, USA.
出版信息
Mol Cell Biol. 2011 Jan;31(1):203-14. doi: 10.1128/MCB.01009-10. Epub 2010 Nov 1.
Abstract
The Pax7 transcription factor is required for muscle satellite cell biogenesis and specification of the myogenic precursor lineage. Pax7 is expressed in proliferating myoblasts but is rapidly downregulated during differentiation. Here we report that miR-206 and -486 are induced during myoblast differentiation and downregulate Pax7 by directly targeting its 3' untranslated region (UTR). Expression of either of these microRNAs in myoblasts accelerates differentiation, whereas inhibition of these microRNAs causes persistence of Pax7 protein and delays differentiation. A microRNA-resistant form of Pax7 is sufficient to inhibit differentiation. Since both these microRNAs are induced by MyoD and since Pax7 promotes the expression of Id2, an inhibitor of MyoD, our results revealed a bistable switch that exists either in a Pax7-driven myoblast state or a MyoD-driven myotube state.
摘要
Pax7 转录因子对于肌肉卫星细胞的发生和生肌前体细胞谱系的特化是必需的。Pax7 在增殖的成肌细胞中表达，但在分化过程中迅速下调。在这里，我们报告说，miR-206 和 miR-486 在成肌细胞分化过程中被诱导，并通过直接靶向其 3'非翻译区 (UTR) 下调 Pax7。这些 microRNAs 中的任何一种在成肌细胞中的表达都加速了分化，而抑制这些 microRNAs 会导致 Pax7 蛋白的持续存在并延迟分化。Pax7 的 microRNA 抗性形式足以抑制分化。由于这两种 microRNAs 都是由 MyoD 诱导的，并且 Pax7 促进了 MyoD 抑制剂 Id2 的表达，我们的结果揭示了一种双稳态开关，它存在于 Pax7 驱动的成肌细胞状态或 MyoD 驱动的肌管状态中。
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本文引用的文献
1
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J Cell Biol. 2010 Sep 6;190(5):867-79. doi: 10.1083/jcb.200911036.
2
Genome-wide MyoD binding in skeletal muscle cells: a potential for broad cellular reprogramming.基因组范围内的 MyoD 在骨骼肌细胞中的结合：广泛细胞重编程的潜力。
Dev Cell. 2010 Apr 20;18(4):662-74. doi: 10.1016/j.devcel.2010.02.014.
3
MicroRNA-206 delays ALS progression and promotes regeneration of neuromuscular synapses in mice.miRNA-206 可延缓肌萎缩侧索硬化症的进展并促进小鼠运动神经元-肌肉突触的再生。
Science. 2009 Dec 11;326(5959):1549-54. doi: 10.1126/science.1181046.
4
miR-24 Inhibits cell proliferation by targeting E2F2, MYC, and other cell-cycle genes via binding to "seedless" 3'UTR microRNA recognition elements.miR-24 通过与“无种子”3'UTR 微小 RNA 识别元件结合，靶向 E2F2、MYC 和其他细胞周期基因来抑制细胞增殖。
Mol Cell. 2009 Sep 11;35(5):610-25. doi: 10.1016/j.molcel.2009.08.020.
5
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J Biol Chem. 2009 Oct 23;284(43):29596-604. doi: 10.1074/jbc.M109.020511. Epub 2009 Aug 26.
6
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7
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8
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Mol Endocrinol. 2009 Aug;23(8):1215-30. doi: 10.1210/me.2009-0062. Epub 2009 May 7.
10
The molecular regulation of muscle stem cell function.肌肉干细胞功能的分子调节
Cold Spring Harb Symp Quant Biol. 2008;73:323-31. doi: 10.1101/sqb.2008.73.064. Epub 2009 Mar 27.
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