Sun Li, Wang Xu
Department of Oncology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
World J Gastroenterol. 2003 Sep;9(9):1930-4. doi: 10.3748/wjg.v9.i9.1930.
To investigate the effects of allicin on both telomerase activity and apoptosis in gastric cancer SGC-7901 cells.
The gastric cancer SGC-7901 adenocarcinoma cells were treated with allicin and the cell cycle, inhibitory rate, apoptosis, telomerase activity and morphologic changes were studied by MTT assay, flow cytometry (FCM), TRAP-PCR-ELISA assay, light microscope, electron microscope respectively. Results were compared with that of AZT (3'-Azido-3'-deoxythymidine).
SGC-7901 cells were suppressed after exposure to allicin of 0.016 mg/ml, 0.05 mg/ml, and 0.1 mg/ml for 48 h. Compared with the control, the difference was significant (P<0.05). Allicin could induce apoptosis of the cells in a dose-dependent and non-linear manner and increase the proportion of cells in the G(2)/M phase. Compared with the control, the difference was significant in terms of the percentage of cells in the G2/M phase (P<0.05). Allicin could inhibit telomerase activity in a time-dependent and dose-dependent pattern. After exposure to allicin at 0.016 mg/ml for 24 hours, SGC-7901 cells showed typical morphologic change.
Allicin can inhibit telomerase activity and induce apoptosis of gastric cancer SGC-7901 cells. Allicin may be more effective than AZT.
研究大蒜素对胃癌SGC - 7901细胞端粒酶活性及细胞凋亡的影响。
用大蒜素处理胃癌SGC - 7901腺癌细胞，分别采用MTT法、流式细胞术（FCM）、端粒重复序列扩增-聚合酶链反应-酶联免疫吸附测定法（TRAP-PCR-ELISA）、光学显微镜及电子显微镜研究细胞周期、抑制率、凋亡、端粒酶活性及形态学变化。将结果与叠氮胸苷（3'-叠氮-3'-脱氧胸苷，AZT）处理组进行比较。
SGC - 7901细胞经0.016 mg/ml、0.05 mg/ml和0.1 mg/ml大蒜素处理48小时后受到抑制。与对照组相比，差异有统计学意义（P<0.05）。大蒜素能以剂量依赖和非线性方式诱导细胞凋亡，并增加G(2)/M期细胞比例。与对照组相比，G2/M期细胞百分比差异有统计学意义（P<0.05）。大蒜素能以时间和剂量依赖模式抑制端粒酶活性。SGC - 7901细胞经0.016 mg/ml大蒜素处理24小时后出现典型形态学改变。
大蒜素可抑制胃癌SGC - 7901细胞端粒酶活性并诱导其凋亡。大蒜素可能比AZT更有效。
