Department of Neurosurgery, Pusan National University Hospital, Pusan National University School of Medicine, Busan 602-739, Republic of Korea.
Oncol Rep. 2012 Jul;28(1):41-8. doi: 10.3892/or.2012.1772. Epub 2012 Apr 23.
Allicin, the main flavor compound in garlic, has anti-carcinogenic activities in a range of cancer cells, however, the underlying molecular mechanisms are not completely understood. This study examined the effect of allicin on the cell viability of U87MG human glioma cells along with its molecular mechanisms of induction of cell death. Apoptosis was determined by TUNEL and Hoechst 33258 staining as well as by western blot analysis. Allicin inhibited the cell viability of U87MG human glioma cells in a dose- and time-dependent manner. Allicin-induced inhibition of cell viability was due to apoptosis of cells. The mechanisms of apoptosis were found to involve the mitochondrial pathway of Bcl-2/Bax, the MAPK/ERK signaling pathway and antioxidant enzyme systems. These results suggest that allicin can serve as a novel chemotherapeutic candidate for the treatment of glioblastoma multiforme.
大蒜中的主要风味化合物蒜素在多种癌细胞中具有抗癌活性，但其中的分子机制尚不完全清楚。本研究探讨了蒜素对 U87MG 人神经胶质瘤细胞活力的影响及其诱导细胞死亡的分子机制。通过 TUNEL 和 Hoechst 33258 染色以及 Western blot 分析来检测细胞凋亡。蒜素呈剂量和时间依赖性地抑制 U87MG 人神经胶质瘤细胞的活力。蒜素诱导的细胞活力抑制是由于细胞凋亡所致。凋亡机制涉及 Bcl-2/Bax 的线粒体途径、MAPK/ERK 信号通路和抗氧化酶系统。这些结果表明，蒜素可以作为治疗多形性胶质母细胞瘤的新型化疗候选药物。
