Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Bioresour Technol. 2013 Aug;142:186-91. doi: 10.1016/j.biortech.2013.04.124. Epub 2013 May 9.
In this study, we show that Sporolactobacillus laevolacticus DSM442 can produce d-lactate by using cottonseed as the sole nitrogen source. The cottonseed was enzymatically hydrolyzed and simultaneously utilized during d-lactate fermentation. Under optimal conditions, a high d-lactic acid concentration (144.4 g/L) was obtained in a fed-batch fermentation within 35 h, with an average productivity of 4.13 g/(Lh) and a yield of 0.96 g/g glucose. The optical purity of d-lactic acid in the broth was 99.3%, which meets the requirement for use in lactic acid polymerization. Our study represents a cost-effective method for polymer-grade d-lactate production using cheap agricultural wastes.
在这项研究中，我们表明，短乳杆菌 DSM442 可以利用棉籽作为唯一的氮源来生产 d-乳酸。棉籽经过酶解，同时在 d-乳酸发酵过程中被利用。在最佳条件下，经过 35 小时的分批补料发酵，获得了 144.4g/L 的高 d-乳酸浓度，平均生产力为 4.13g/（Lh），葡萄糖得率为 0.96g/g。发酵液中 d-乳酸的光学纯度为 99.3%，符合用于乳酸聚合的要求。我们的研究代表了一种使用廉价农业废弃物生产聚合物级 d-乳酸的具有成本效益的方法。
