Universidade Federal do Rio de Janeiro, Departamento de Química Orgânica, Rio de Janeiro, RJ, Brazil.
Phytochem Anal. 2010 Nov-Dec;21(6):539-43. doi: 10.1002/pca.1229.
Phytochemical and biological studies carried out on Copaifera species showed that their oleoresins and isolated compounds have various biological activities.
The aims of this work were (i) to analyse the Copaifera oleoresin by gas chromatography-mass spectrometry, (ii) to isolate the diterpenic acids from this oleoresin by high-speed countercurrent chromatography (HSCCC) and (iii) to determine the rhodamine 6G Pdr5p activity of these acids.
HSCCC was used for the preparative separation of the diterpenes. Spectroscopic methods were used to establish their identity.
The gas chromatogram of the oleoresin showed approximately 30 compounds. The two major ones, kaur-16-en-18-oic and polyalthic acids, were isolated in high purity. Kaur-16-en-18-oic acid exhibited the highest rodomine 6G Pdr5p activity among the tested compounds.
HSCCC was shown to be a quick and effective tool in the isolation and purification of diterpenes from Copaifera oleoresin. This is the first report on the use of HSCCC for the fractionation of an oleoresin from Copaifera and the isolation of diterpenes therein.
对 Copaifera 属植物进行的植物化学和生物研究表明，其油树脂和分离出的化合物具有多种生物活性。
本工作的目的是 (i) 通过气相色谱-质谱法分析 Copaifera 油树脂，(ii) 通过高速逆流色谱 (HSCCC) 从该油树脂中分离二萜酸，以及 (iii) 测定这些酸对吖啶橙 6G Pdr5p 的活性。
HSCCC 用于二萜的制备分离。光谱方法用于确定它们的身份。
油树脂的气相色谱图显示了大约 30 种化合物。两种主要成分，贝壳杉-16-烯-18-酸和多立特酸，以高纯度分离。在测试的化合物中，贝壳杉-16-烯-18-酸对吖啶橙 6G Pdr5p 的活性最高。
HSCCC 被证明是从 Copaifera 油树脂中分离和纯化二萜的快速有效工具。这是首次报道使用 HSCCC 对 Copaifera 的油树脂进行分离，并从中分离出二萜。
