Lipid Research Unit, Rambam Medical Center and the Bruce Rappaport Faculty of Medicine and Department of Food Engineering and Biotechnology, Technion-Israel Institute of Technology, Haifa, Israel.
Platelets. 1994;5(3):175-6. doi: 10.3109/09537109409005532.
Platelet lipid composition was determined in subjects with hypercholesterolemia (HC) (9.17 ± 2.15 mmol/L), hypocholesterolemia (HYPOC) (3.29 ± 1.01 mmol/L), and normocholesterolemic controls (NC) (5.29 ± 0.82 mmol/L). Lipid composition was quantitated in washed platelets by measuring platelet cholesterol (C) and phospholipid (PL) content, C/PL molar ratio and platelet PL phosphatidylcholine (PC) to sphingmyelin (SM) ratio. There was a significant increase and a significant decrease in C/PL molar ratio in subjects with HC and HYPOC compared to normals (0.74 ± 0.06 and 0.53 ± 0.06 vs 0.59 ± 0.04, p<0.01, respectively). These alterations were due to a significant change in platelet C content, whereas, platelet PL content was stable. PC/SM ratio was markedly decreased in HYPOC compared to NC (0.80 ± 0.18 vs 2.03 ± 0.18, p<0.01, respectively). These results indicate that blood cholesterol is a major determinant of platelet membrane lipid composition. This effect may be of concern in relation to a possible excess morbidity in patients not only with HC, but also with a low blood cholesterol.
在高胆固醇血症（HC）患者（9.17 ± 2.15 mmol/L）、低胆固醇血症（HYPOC）患者（3.29 ± 1.01 mmol/L）和正常胆固醇血症对照组（NC）（5.29 ± 0.82 mmol/L）中确定血小板脂质组成。通过测量血小板胆固醇（C）和磷脂（PL）含量、C/PL 摩尔比以及血小板 PL 磷脂酰胆碱（PC）与鞘磷脂（SM）的比值，对洗涤后的血小板中的脂质组成进行定量分析。与正常对照组相比，HC 和 HYPOC 患者的 C/PL 摩尔比显著增加和显著降低（0.74 ± 0.06 和 0.53 ± 0.06 与 0.59 ± 0.04，分别为 p<0.01）。这些改变是由于血小板 C 含量的显著变化，而血小板 PL 含量保持稳定。与 NC 相比，HYPOC 患者的 PC/SM 比值显著降低（0.80 ± 0.18 与 2.03 ± 0.18，分别为 p<0.01）。这些结果表明，血液胆固醇是血小板膜脂质组成的主要决定因素。这种影响可能与不仅患有高胆固醇血症，而且还患有低胆固醇血症的患者可能出现的发病率增加有关。
