Lipid Research Unit, Rambam Medical Center, Haifa, and the Bruce Rappaport Faculty of Medicine and Department of Food Engineering and Biotechnology, Technion-Israel Institute of Technology, Haifa, Israel.
Platelets. 1992;3(1):57-9. doi: 10.3109/09537109209013170.
Aspirin, 250 mg/day, was administered to 14 normolipidemic healthy subjects for 7 days. Platelet lipid composition was determined in washed platelets by quantitating cholesterol to phospholipid molar ratio. Platelet membrane fluidity was measured by steady state fluorescence polarization using the probe diphenyl hexatriene. Upon 7 days aspirin ingestion platelet lipid composition was not altered. There was a sex-dependent effect of aspirin administration on platelet membrane fluidity. Whereas overall platelet membrane fluidity did not change at 37°C, there was a significant decrease in female subjects; the anisotropy parameter which is inversely related to membrane fluidity increased from 0.937±0.043-1.048 ±0.027 (p<0.01). In male subjects there was an increase in platelet membrane fluidity, which was significant at 25° C; the anisotropy parameter decreased from 1.350 ± 0.039-1.283±0.023 (p < 0.05). These results indicate that aspirin alters the membrane dynamics of platelets. This effect results from mechanisms other than alterations in platelet cholesterol or phospholipid content and operates in an opposite direction in men and women.
阿司匹林，250 毫克/天，给予 14 例血脂正常的健康受试者，共 7 天。通过定量测定胆固醇与磷脂的摩尔比，在洗涤后的血小板中测定血小板的脂质组成。使用探针二苯己烯来测量血小板膜的流动性，通过稳态荧光偏振来测定。在服用 7 天后，阿司匹林未改变血小板的脂质组成。阿司匹林给药对血小板膜流动性有性别依赖性的影响。尽管在 37°C 时整体血小板膜流动性没有变化，但女性受试者的流动性显著降低；各向异性参数（与膜流动性成反比）从 0.937±0.043-1.048 ±0.027（p<0.01）。在男性受试者中，血小板膜流动性增加，在 25°C 时显著增加；各向异性参数从 1.350 ± 0.039-1.283±0.023（p < 0.05）降低。这些结果表明阿司匹林改变了血小板的膜动力学。这种作用是由于除了血小板胆固醇或磷脂含量的改变以外的机制引起的，并且在男性和女性中的作用方向相反。
