Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, Milan, Italy.
Curr Pharm Biotechnol. 2011 Feb 1;12(2):146-50. doi: 10.2174/138920111794295828.
Generation of pluripotent stem cells (iPSCs) from adult fibroblasts starts a "new era" in stem cell biology, as it overcomes several key issues associated with previous approaches, including the ethical concerns associated with human embryonic stem cells. However, as the genetic approach for cell reprogramming has already shown potential safety issues, a chemical approach may be a safer and easier alternative. Moreover, a chemical approach could be advantageous not only for the de-differentiation phase, but also for inducing reprogrammed cells into the desired cell type with higher efficiency than current methodologies. Finally, a chemical approach may be envisioned to activate resident adult stem cells to proliferate and regenerate damaged tissues in situ, without the need for exogenous cell injections.
从成纤维细胞中生成多能干细胞（iPSCs）开创了干细胞生物学的“新纪元”，因为它克服了与以前方法相关的几个关键问题，包括与人类胚胎干细胞相关的伦理问题。然而，由于细胞重编程的遗传方法已经显示出潜在的安全问题，化学方法可能是一种更安全、更容易的替代方法。此外，化学方法不仅在去分化阶段，而且在以比当前方法更高的效率将重编程细胞诱导为所需细胞类型方面，都可能具有优势。最后，可以设想使用化学方法来激活驻留的成年干细胞，使其在原位增殖并再生受损组织，而无需外源细胞注射。
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