一种用于测定益母草（Leonurus japonicus）中益母草碱的超高效液相色谱-二极管阵列检测器-质谱联用方法的开发与验证
Development and validation of an UPLC-DAD-MS method for the determination of leonurine in Chinese motherwort (Leonurus japonicus).
作者信息
Chen Zhe, Wu Jian Bing, Liao Xiao Juan, Yang Wei, Song Kang
机构信息
National Clinical Research Base of Traditional Chinese Medicine, Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, Hangzhou 310006, China.
出版信息
J Chromatogr Sci. 2010 Nov;48(10):802-6. doi: 10.1093/chromsci/48.10.802.
In the present study, an ultra-performance liquid chromatography method with diode array detection (UPLC-DAD) was developed for the analysis and determination of leonurine from motherwort (Leonurus japonicus), a traditional Chinese medicinal plant. This method was validated in terms of specificity, linearity (R(2) = 0.9995, linear range: 0.005 ~ 0.5 mg/mL), precision (< 5.0% RSD), and recovery (103.2%). The extracted amount of leonurine is 0.15 mg/g. Moreover, the target analyte was identified or tentatively characterized using UPLC coupled with electrospary tandem mass spectrometry (UPLC-ESI-MS).
在本研究中，开发了一种采用二极管阵列检测的超高效液相色谱法（UPLC-DAD），用于分析和测定传统中药益母草（Leonurus japonicus）中的益母草碱。该方法在特异性、线性（R(2) = 0.9995，线性范围：0.005 ~ 0.5 mg/mL）、精密度（< 5.0% RSD）和回收率（103.2%）方面得到了验证。益母草碱的提取量为0.15 mg/g。此外，使用超高效液相色谱与电喷雾串联质谱联用（UPLC-ESI-MS）对目标分析物进行了鉴定或初步表征。
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