Fattahi Azin, Shakeri Abolfazl, Tayarani-Najaran Zahra, Kharbach Mourad, Segers Karen, Heyden Yvan Vander, Taghizadeh Seyedeh Faezeh, Rahmani Hanieh, Asili Javad
Department of Pharmacognosy School of Pharmacy Mashhad University of Medical Sciences Mashhad Iran.
Department of Pharmacodynamics and Toxicology School of Pharmacy Mashhad University of Medical Sciences Mashhad Iran.
Food Sci Nutr. 2021 Jun 14;9(8):4278-4286. doi: 10.1002/fsn3.2396. eCollection 2021 Aug.
L. is a significant component in the Iranian food basket. This study aimed to investigate the bioactive compounds and biological activities of different extracts obtained from aerial parts. From the aerial parts, a crude methanolic (MeOH) extract and its four sub-fractions, that is, petroleum ether (Pet), ethyl acetate (EtOAc), n-butanol (n-BuOH), and aqueous (water) extracts were obtained. The total phenolic and flavonoid contents as well as the antioxidant and cytotoxic activities of the extracts were determined. Moreover, the phytochemical profiles of the essential oil (EO) and of those extracts with the highest antioxidant activity measured by GC/MS and UPLC-PDA-ESI-QTOF-MS/MS. Results showed that the highest concentrations of phenols and flavonoids as well as the most potent antioxidant potential according to the DPPH method were determined in the EtOAc and MeOH extracts with IC values of 22.0 and 34.4 µg.ml, respectively. Quantitative analysis of these extracts was subsequently performed by UPLC-PDA-ESI-QTOF-MS/MS. Both extracts contained mainly rosmarinic acid, caffeic acid, and 2-hydroxycinnamic acid, which may be responsible for their high antioxidant activity. Moreover, none of the extracts showed cytotoxic effects against MCF7, SW48, and a normal cell line of mouse embryonic fibroblast cells (NIH/3T3) in the tested concentrations (up to 400 μg.ml). Additionally, GC-MS analysis showed that oxygenated monoterpenes (55.4%) were the main constituents of the EO of .
L. 是伊朗食物篮子中的一个重要组成部分。本研究旨在调查从地上部分获得的不同提取物的生物活性化合物和生物活性。从地上部分获得了粗甲醇（MeOH）提取物及其四个亚组分，即石油醚（Pet）、乙酸乙酯（EtOAc）、正丁醇（n-BuOH）和水提取物。测定了提取物的总酚和黄酮含量以及抗氧化和细胞毒性活性。此外，通过气相色谱/质谱联用仪（GC/MS）和超高效液相色谱-光电二极管阵列-电喷雾电离-四极杆飞行时间串联质谱仪（UPLC-PDA-ESI-QTOF-MS/MS）测定了精油（EO）和抗氧化活性最高的那些提取物的植物化学图谱。结果表明，根据二苯基苦味酰基自由基（DPPH）法，在乙酸乙酯和甲醇提取物中测定出最高浓度的酚类和黄酮类化合物以及最强的抗氧化潜力，其半数抑制浓度（IC）值分别为22.0和34.4 μg.ml。随后通过UPLC-PDA-ESI-QTOF-MS/MS对这些提取物进行了定量分析。两种提取物主要含有迷迭香酸、咖啡酸和2-羟基肉桂酸，这可能是它们具有高抗氧化活性的原因。此外，在所测试的浓度（高达400 μg.ml）下，没有一种提取物对MCF7、SW48和小鼠胚胎成纤维细胞正常细胞系（NIH/3T3）显示出细胞毒性作用。另外，气相色谱-质谱联用（GC-MS）分析表明，氧化单萜（55.4%）是L. 精油的主要成分。
