Department of Anatomy and Cell Biology, University of Illinois-Chicago, Chicago, IL 60612-7308, USA.
Brain Res. 2011 Jan 19;1369:10-20. doi: 10.1016/j.brainres.2010.10.092. Epub 2010 Oct 31.
In the developing PNS, axonal neuregulin-1 (NRG1) type III is the key determinant for myelination. However, the specific role for NRG1 (III) in the CNS has not been established. To address this issue, isotype-specific antibodies were generated, characterized, and used for the immunofluorescent localization of NRG1 (III) in the developing and adult CNS of rat. In contrast to adult peripheral nerve, which showed robust axonal staining, no immunoreactivity was observed in CNS myelinated tracts during the period of active myelination or in the adult CNS. Surprisingly, NRG1 (III) was prominently expressed on dendrites and soma in both the developing and adult CNS. These findings were corroborated through the subcellular fractionation of adult rat brain combined with an immunoblotting analysis. The immunolocalization of NRG1 (III) suggests that it plays a novel role in the myelination fate of CNS axons possibly through undetermined roles in neuronal maturation, or dendritic development and activation.
在发育中的周围神经系统中，轴突神经调节蛋白 1（NRG1）III 型是髓鞘形成的关键决定因素。然而，NRG1（III）在中枢神经系统中的具体作用尚未确定。为了解决这个问题，生成了同种型特异性抗体，并对其进行了表征，用于免疫荧光定位大鼠发育中和成年中枢神经系统中的 NRG1（III）。与成年周围神经中强烈的轴突染色形成鲜明对比的是，在活跃的髓鞘形成期间或在成年中枢神经系统中，未观察到中枢神经系统髓鞘化束中有免疫反应性。令人惊讶的是，NRG1（III）在发育中和成年中枢神经系统中的树突和体上都有明显表达。这些发现通过结合免疫印迹分析对成年大鼠脑的亚细胞级分进行了证实。NRG1（III）的免疫定位表明，它可能在中枢神经系统轴突的髓鞘形成命运中发挥新的作用，可能通过在神经元成熟、树突发育和激活方面的不确定作用。
