Department of Stomatology, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China.
Biomed Res Int. 2013;2013:438243. doi: 10.1155/2013/438243. Epub 2013 Jul 7.
Both human adipose tissue-derived mesenchymal stem cells (ASCs) and umbilical cord-derived mesenchymal stem cells (UC-MSCs) have been explored as attractive mesenchymal stem cells (MSCs) sources, but very few parallel comparative studies of these two cell types have been made. We designed a side-by-side comparative study by isolating MSCs from the adipose tissue and umbilical cords from mothers delivering full-term babies and thus compared the various biological aspects of ASCs and UC-MSCs derived from the same individual, in one study. Both types of cells expressed cell surface markers characteristic of MSCs. ASCs and UC-MSCs both could be efficiently induced into adipocytes, osteoblasts, and neuronal phenotypes. While there were no significant differences in their osteogenic differentiation, the adipogenesis of ASCs was more prominent and efficient than UC-MSCs. In the meanwhile, ASCs responded better to neuronal induction methods, exhibiting the higher differentiation rate in a relatively shorter time. In addition, UC-MSCs exhibited a more prominent secretion profile of cytokines than ASCs. These results indicate that although ASCs and UC-MSCs share considerable similarities in their immunological phenotype and pluripotentiality, certain biological differences do exist, which might have different implications for future cell-based therapy.
人脂肪组织来源间充质干细胞（ASCs）和脐带间充质干细胞（UC-MSCs）均被探索为有吸引力的间充质干细胞（MSCs）来源，但对这两种细胞类型的平行比较研究很少。我们通过从足月分娩的母亲的脂肪组织和脐带中分离出 MSC 设计了一项并列比较研究，从而在一项研究中比较了来自同一个体的 ASC 和 UC-MSC 的各种生物学方面。这两种类型的细胞都表达 MSC 的特征性细胞表面标志物。ASC 和 UC-MSCs 均能有效地被诱导分化为成脂细胞、成骨细胞和神经元表型。虽然它们的成骨分化没有显著差异，但 ASC 的成脂分化更为显著和高效。同时，ASC 对神经元诱导方法的反应更好，在相对较短的时间内表现出更高的分化率。此外，UC-MSCs 的细胞因子分泌谱比 ASC 更显著。这些结果表明，尽管 ASC 和 UC-MSCs 在免疫表型和多能性方面具有相当大的相似性，但确实存在某些生物学差异，这可能对未来的基于细胞的治疗有不同的影响。
