Department of Internal Medicine, School of Medicine, Pusan National University, 626-870 Busan, Republic of Korea.
Exp Parasitol. 2011 Feb;127(2):539-44. doi: 10.1016/j.exppara.2010.10.004. Epub 2010 Oct 30.
In an effort to define the mechanism underlying the host immune downregulation inherent to Trichinella spiralis infection, we compared the levels of Th1, Th2, and regulatory cytokines and CD4(+)CD25(+) forkhead box P3 (FoxP3)(+) T (T(reg)) cell recruitment, as well as cellular pathology in the airway between T. spiralis infected and uninfected asthma-induced mice. After the induction of allergic airway inflammation, we noted influxes of inflammatory cells into the peribronchial tree. However, in the T. spiralis infection groups, cellular infiltration was minimal around the bronchial tree, with only a smattering of inflammatory cells. In the OVA-challenged group after T. spiralis infection, the numbers of macrophages and eosinophils in the bronchial alveolar lavage fluid were reduced by 23% and 52%, respectively, as compared to those of the OVA-challenged group. Airway hyperresponsiveness of OVA-challenged mice after T. spiralis infection was significantly suppressed as compared to the OVA-only challenged mice. The T. spiralis-infected mice exhibited a significant reduction in IL-5 concentrations relative to that noted in the OVA-challenged group (p<0.01). Nevertheless, the regulatory cytokines IL-10 and TGF-β levels were increased significantly as the result of T. spiralis infection, and we verified the recruitment of T(reg) cells in lung draining lymph nodes via T. spiralis infection. Therefore, T(reg) cells, which were recruited by T. spiralis infection, might ameliorate lung function and reduce allergic airway inflammation.
为了阐明旋毛虫感染所固有宿主免疫下调的机制，我们比较了 Th1、Th2 和调节性细胞因子的水平，以及 CD4+CD25+叉头框 P3（FoxP3）+T（Treg）细胞募集情况，以及感染和未感染哮喘诱导的旋毛虫感染小鼠气道中的细胞病理学。在诱导过敏性气道炎症后，我们观察到炎症细胞流入支气管树周围。然而，在旋毛虫感染组中，支气管树周围的细胞浸润最小，仅有少量炎症细胞。在旋毛虫感染后的 OVA 挑战组中，与 OVA 挑战组相比，支气管肺泡灌洗液中的巨噬细胞和嗜酸性粒细胞数量分别减少了 23%和 52%。与仅 OVA 挑战组相比，旋毛虫感染后的 OVA 挑战小鼠的气道高反应性显著受到抑制。与 OVA 挑战组相比，旋毛虫感染小鼠的 IL-5 浓度显著降低（p<0.01）。然而，由于旋毛虫感染，调节性细胞因子 IL-10 和 TGF-β 水平显著升高，并且我们通过旋毛虫感染证实了肺引流淋巴结中 Treg 细胞的募集。因此，旋毛虫感染募集的 Treg 细胞可能改善肺功能并减轻过敏性气道炎症。
