Department of Pathology, University of Michigan, Ann Arbor, MI, USA.
Semin Nephrol. 2010 Sep;30(5):455-67. doi: 10.1016/j.semnephrol.2010.07.003.
The sum of RNA transcripts of a cell, organ structure, or organism can be referred to as transcriptome. An increasing number of studies report on specific and common alterations in the renal transcriptome in human nephropathies. In this review several challenges in transcriptomic analyses of the human kidney are discussed. This includes ways to approach the heterogeneity of the kidney itself as well as the diversity of renal diseases. Conventional and upcoming techniques for transcriptional profiling of minute tissue samples are presented, including so-called next generation sequencing and microRNA detection. Different tools to integrate transcriptomic data in a systematic context are discussed beside the current challenge to combine such results with data sets from other integrative biology technologies.
细胞、器官结构或生物体的 RNA 转录本的总和可以被称为转录组。越来越多的研究报告了人类肾病中肾脏转录组的特定和常见改变。在这篇综述中，讨论了人类肾脏转录组分析中的几个挑战。这包括如何处理肾脏本身的异质性以及肾脏疾病的多样性。介绍了常规和即将出现的用于微小组织样本转录谱分析的技术，包括所谓的下一代测序和 microRNA 检测。除了当前将这些结果与其他综合生物学技术的数据集相结合的挑战外，还讨论了在系统环境中整合转录组数据的不同工具，以及将这些结果与其他综合生物学技术的数据集相结合的挑战。
