Zürbig Petra, Schiffer Eric, Mischak Harald
Mosaiques diagnostics GmbH, Mellendorfer-Str. 7-9, 30625 Hannover, Germany.
Methods Mol Biol. 2009;564:105-21. doi: 10.1007/978-1-60761-157-8_6.
Currently, the main focus of clinical proteome analysis is on detection and identification of polypeptides that significantly change owing to pathological changes. Capillary electrophoresis coupled online to an electrospray ionization time of flight mass spectrometer (CE-MS) allows the differential display of a large number of polypeptides in a single, reproducible, and time-limited step and enables the comparison of different protein profiles for biomarker discovery. In addition to the reproducibility of the CE-MS setup, many further steps including data processing and mining, usage of biomarkers for diagnosis, and biomarker sequencing are necessary to answer the demands of biomarker discovery of clinical significance. In this chapter, we discuss materials and methods for CE-MS-based clinical proteomics allowing the reproducible profiling of urine.
目前，临床蛋白质组分析的主要重点是检测和鉴定因病理变化而显著改变的多肽。毛细管电泳与电喷雾电离飞行时间质谱仪（CE-MS）在线联用，能够在一个单一、可重复且限时的步骤中对大量多肽进行差异显示，并能够比较不同的蛋白质谱以发现生物标志物。除了CE-MS设置的可重复性之外，还需要许多其他步骤，包括数据处理与挖掘、将生物标志物用于诊断以及生物标志物测序，以满足具有临床意义的生物标志物发现的需求。在本章中，我们将讨论基于CE-MS的临床蛋白质组学的材料和方法，这些方法可实现尿液的可重复分析。
