National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
Chemosphere. 2011 Jan;82(1):56-63. doi: 10.1016/j.chemosphere.2010.10.009. Epub 2010 Nov 1.
Four microbial species (Kocuria rhizophila, Microbacterium resistens, Staphylococcus equorum and Staphylococcus cohnii subspecies urealyticus) were isolated from the rhizospheric zone of selected plants growing in a lindane contaminated environment and acclimatized in lindane spiked media (5-100 μg mL⁻¹). The isolated species were inoculated with soil containing 5, 50 and 100 mg kg⁻¹ of lindane and incubated at room temperature. Soil samples were collected periodically to evaluate the microbial dissipation kinetics, dissipation rate, residual lindane concentration and microbial biomass carbon (MBC). There was a marked difference (p < 0.05) in the MBC content and lindane dissipation rate of microbial isolates cultured in three different lindane concentrations. Further, the dissipation rate tended to decrease with increasing lindane concentrations. After 45 d, the residual lindane concentrations in three different spiked soils were reduced to 0%, 41% and 33%, respectively. Among the four species, S. cohnii subspecies urealyticus exhibited maximum dissipation (41.65 mg kg⁻¹) and can be exploited for the in situ remediation of low to medium level lindane contaminated soils.
从生长在林丹污染环境中的选定植物的根际区分离出了四种微生物物种（玫瑰色微球菌、抵抗微杆菌、马厩葡萄球菌和解脲葡萄球菌亚种），并在林丹污染的培养基（5-100μg/mL）中驯化。将分离出的物种接种到含有 5、50 和 100mg/kg 林丹的土壤中，并在室温下培养。定期采集土壤样本，以评估微生物的消解动力学、消解速率、残留林丹浓度和微生物生物量碳（MBC）。在三种不同林丹浓度下培养的微生物分离物的 MBC 含量和林丹消解速率有明显差异（p<0.05）。此外，消解速率随着林丹浓度的增加而降低。45 天后，三种不同添加土壤中的残留林丹浓度分别降低到 0%、41%和 33%。在这四种物种中，解脲葡萄球菌亚种表现出最大的消解（41.65mg/kg），可用于原位修复低至中等水平林丹污染土壤。
