比较屠宰猪肌肉液和血清中抗戊型肝炎病毒抗体的检测。
Comparison of muscle fluid and serum for detection of antibodies against hepatitis E virus in slaughter pigs.
机构信息
Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
出版信息
Vet J. 2011 Oct;190(1):179-80. doi: 10.1016/j.tvjl.2010.09.030. Epub 2010 Nov 1.
Muscle fluid was investigated as an alternative to serum for detecting anti-hepatitis E virus (HEV) antibodies in slaughter pigs. Samples of serum and diaphragmatic muscle juice from 67 pigs were analysed by anti-HEV IgG ELISA. Compared to the serum ELISA, the ELISA on diaphragmatic muscle fluid had a sensitivity of 93%, a specificity of 91.7%, a κ value of 0.84 and a determination coefficient (R(2)) of 0.77; both tests had global agreement of results. Muscle fluid can be used as an alternative to serum for serological detection of HEV antibodies in slaughter pigs.
肌肉液被研究作为血清的替代物，用于检测屠宰猪中的抗戊型肝炎病毒（HEV）抗体。通过抗-HEV IgG ELISA 分析了 67 头猪的血清和膈肌肌肉液样本。与血清 ELISA 相比，膈肌肌肉液 ELISA 的敏感性为 93%，特异性为 91.7%，κ 值为 0.84，决定系数（R²）为 0.77；两种检测方法的结果均具有全球一致性。肌肉液可以作为血清的替代物，用于屠宰猪中 HEV 抗体的血清学检测。
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