Tu Z H, Wang M Y, Xiao W Q, Qi X D, Tang W D
Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
Zhongguo Yao Li Xue Bao. 1990 Jul;11(4):378-81.
10-Hydroxycamptothecin (HC) is a new antitumor principle isolated from Camptotheca acuminata indigenous to China. The genetic toxicity of HC was assessed by mouse bone marrow and transplacental micronucleus test as well as Chinese hamster ovary cell chromosomal aberrations. All of these tests showed positive results. The highest rate of chromosomal aberrations was 83% at 0.125 microgram/ml for 48 h. The number of micronucleated polychromatic erythrocytes in bone marrow of mice was remarkably increased in 19.8% cells at 12.5 mg/kg for 24 h. The micronucleus formation was most often seen at 16 h after im HC in fetal liver and 24 h in maternal bone marrow. The peaks were 36 +/- 19 and 31 +/- 10%, respectively. The results from in vivo and in vitro suggest HC is a mutagen, furthermore, a transplacental mutagen in mouse.
10-羟基喜树碱（HC）是从中国本土的喜树中分离出的一种新型抗肿瘤成分。通过小鼠骨髓和经胎盘微核试验以及中国仓鼠卵巢细胞染色体畸变试验对HC的遗传毒性进行了评估。所有这些试验均显示出阳性结果。在0.125微克/毫升处理48小时时，染色体畸变的最高发生率为83%。在12.5毫克/千克处理24小时时，小鼠骨髓中微核多染红细胞的数量在19.8%的细胞中显著增加。在给胎儿肝脏腹腔注射HC后16小时以及给母体骨髓注射后24小时，微核形成最为常见。峰值分别为36±19%和31±10%。体内和体外试验结果表明，HC是一种诱变剂，此外，在小鼠中还是一种经胎盘诱变剂。
