Cell Biology Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, University Walk, Bristol, BS8 1TD, UK.
J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8. doi: 10.1242/jcs.076000. Epub 2010 Nov 2.
Mitotic inhibition of COPII-dependent export of proteins from the endoplasmic reticulum results in disassembly of the Golgi complex. This ensures ordered inheritance of organelles by the two daughter cells. Reassembly of the Golgi is intimately linked to the re-initiation of ER export on exit from mitosis. Here, we show that unlike all other COPII components, which are cytosolic during metaphase, Sec16A remains associated with ER exit sites throughout mitosis, and thereby could provide a template for the rapid assembly of functional export domains in anaphase. Full assembly of COPII at exit sites precedes reassembly of the Golgi in telophase.
有丝分裂抑制 COPII 依赖性内质网中蛋白质的输出，导致高尔基体复合体解体。这确保了细胞器被两个子细胞有序地继承。高尔基体的重新组装与有丝分裂后 ER 输出的重新开始密切相关。在这里，我们表明，与所有其他在有丝分裂中期存在于细胞质中的 COPII 成分不同，Sec16A 在整个有丝分裂过程中仍然与 ER 出口部位结合，从而可以为后期功能性出口结构域的快速组装提供模板。COPII 在出口部位的完全组装先于末期高尔基体的重新组装。
