Department of Nephrology, Affiliated the No.1 People's Hospital of Huaian, Nanjing Medical University, Huaian, Jiangsu, PR China.
Dis Markers. 2010;29(2):89-93. doi: 10.3233/DMA-2010-0730.
The development of cardiovascular disease in ESRD patients is considered to be associated with oxidative stress. NAD(P)H oxidase has attracted attention as mechanisms of generating oxidative stress. We investigated the relation between the genotype of the C242T CYBA polymorphism of the NADPH oxidase and the development of cardiovascular disease in ESRD patients.
A total of 289 ESRD patients were recruited and allocated to one of the two groups: patients without cardiovascular disease (group N; n=192) and patients developing cardiovascular disease (group D; n=97). The C242T CYBA polymorphism was determined by RFLP-PCR methods.
The frequency of the C242T CT+TT genotype was significantly lower in group D than in group N (9.1 vs. 20.2%). In multiple Logistic regression analysis, systolic blood pressure, smoking history and this gene polymorphism were shown to be independent variables for the development of cardiovascular disease in ESRD patients.
These results suggest that assessment of the C242T CYBA polymorphism of the NADPH oxidase may be useful in identifying the risk for developing cardiovascular disease in ESRD patients.
ESRD 患者心血管疾病的发展被认为与氧化应激有关。NAD(P)H 氧化酶作为产生氧化应激的机制引起了关注。我们研究了 NADPH 氧化酶的 C242T CYBA 多态性的基因型与 ESRD 患者心血管疾病发展之间的关系。
共招募了 289 名 ESRD 患者，并将其分为两组：无心血管疾病患者（组 N；n=192）和发生心血管疾病患者（组 D；n=97）。采用 RFLP-PCR 方法确定 C242T CYBA 多态性。
组 D 的 C242T CT+TT 基因型频率明显低于组 N（9.1%比 20.2%）。在多因素 Logistic 回归分析中，收缩压、吸烟史和该基因多态性是 ESRD 患者发生心血管疾病的独立变量。
这些结果表明，评估 NADPH 氧化酶的 C242T CYBA 多态性可能有助于识别 ESRD 患者发生心血管疾病的风险。
