Department of Pathology and Laboratory Medicine, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pharmacology & Toxicology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
Eur J Clin Invest. 2020 Sep;50(9):e13275. doi: 10.1111/eci.13275. Epub 2020 Jul 29.
Both inflammation and oxidative stress may contribute to pathogenesis of metabolic syndrome (MetS). The C242T polymorphism (rs4673) in the CYBA gene, as the main components of NAD (P) H oxidase, causes inter-individual variability in the enzyme activity. We aimed to investigate the association between this polymorphism with MetS and its components.
Two hundred nine patients with MetS and 232 controls were included in this study. MetS was defined based on NCEP ATP-III A criteria with some modifications. The C242T polymorphism within CYBA gene was determined by using PCR-based restriction fragment length polymorphism (PCR-RFLP) method.
After applying a multiple logistic regression model with adjusting for potential confounders of MetS including, age, sex, body mass index, hypertension, used medications, and diabetes mellitus, C242T polymorphism was found to be associated with the presence of MetS in men but not in the total population or in women. T allele as compared to C allele was associated with decreased odds of MetS in men (adjusted OR = 0.42, 95% CI = 0.24-0.74; P = .003), but not in women (adjusted OR = 1.03, 95% CI = 0.07-1.61; P = .890), or in the total population (adjusted OR = 0.72, 95% CI = 0.51-1.02; P = .063).
This study shows that T allele of C242T polymorphism in CYBA gene is protective against MetS in Iranian men but not in women. Further cohort studies with larger sample size in subgroups of men and women are required to confirm such association in other racial or ethnic group.
炎症和氧化应激都可能导致代谢综合征（MetS）的发病机制。CYBA 基因中的 C242T 多态性（rs4673）是 NAD（P）H 氧化酶的主要组成部分，导致个体间酶活性存在差异。我们旨在研究该多态性与 MetS 及其成分的关系。
本研究纳入了 209 例 MetS 患者和 232 例对照者。MetS 的定义是根据 NCEP ATP-III A 标准，并进行了一些修改。使用基于聚合酶链反应的限制性片段长度多态性（PCR-RFLP）方法确定 CYBA 基因内的 C242T 多态性。
在调整了 MetS 的潜在混杂因素，包括年龄、性别、体重指数、高血压、用药和糖尿病后，应用多元逻辑回归模型发现，C242T 多态性与男性而不是总人群或女性的 MetS 存在相关。与 C 等位基因相比，T 等位基因与男性 MetS 的发生几率降低相关（校正 OR=0.42，95%CI=0.24-0.74；P=0.003），但在女性（校正 OR=1.03，95%CI=0.07-1.61；P=0.890）或总人群中（校正 OR=0.72，95%CI=0.51-1.02；P=0.063）并非如此。
本研究表明，CYBA 基因 C242T 多态性的 T 等位基因对伊朗男性的 MetS 具有保护作用，但对女性则没有。需要在男性和女性亚组中进行具有更大样本量的队列研究，以在其他种族或族群中证实这种关联。
