Bonafé Nathalie, Zhan Bin, Bottazzi Maria Elena, Perez Oriana A, Koski Raymond A, Asojo Oluwatoyin A
L2 Diagnostics LLC, 300 George Street, New Haven, CT 06511, USA.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Nov 1;66(Pt 11):1487-9. doi: 10.1107/S1744309110035669. Epub 2010 Oct 28.
Glioma pathogenesis-related protein 1 (GLIPR1) is a member of the CAP superfamily that includes proteins from a wide range of eukaryotic organisms. The biological functions of most CAP proteins, including GLIPR1, are unclear. GLIPR1 is up-regulated in aggressive glioblastomas and contributes to the invasiveness of cultured glioblastoma cells. In contrast, decreased GLIPR1 expression is associated with advanced prostate cancer. Forced GLIPR1 overexpression is pro-apoptotic in prostate cancer cells and is being tested in clinical trials as an experimental prostate-cancer therapy. Human GLIPR1 was expressed as a truncated soluble protein (sGLIPR1), purified and crystallized. Useful X-ray data have been collected to beyond 1.9 Å resolution from a crystal that belonged to the orthorhombic space group P2(1)2(1)2 with average unit-cell parameters a = 85.1, b = 79.5, c = 38.9 Å and either a monomer or dimer in the asymmetric unit.
胶质瘤发病相关蛋白1（GLIPR1）是CAP超家族的成员之一，该超家族包含来自多种真核生物的蛋白质。包括GLIPR1在内的大多数CAP蛋白的生物学功能尚不清楚。GLIPR1在侵袭性胶质母细胞瘤中上调，并促进培养的胶质母细胞瘤细胞的侵袭性。相比之下，GLIPR1表达降低与晚期前列腺癌相关。强制GLIPR1过表达在前列腺癌细胞中具有促凋亡作用，目前正在作为一种实验性前列腺癌治疗方法进行临床试验。人GLIPR1表达为截短的可溶性蛋白（sGLIPR1），经过纯化和结晶。已从属于正交空间群P2(1)2(1)2的晶体收集到分辨率超过1.9 Å的有用X射线数据，其平均晶胞参数为a = 85.1、b = 79.5、c = 38.9 Å，不对称单元中存在单体或二聚体。
