School of Pharmacy, University of Otago , Dunedin , New Zealand ; Department of Pathology, Dunedin School of Medicine, University of Otago , Dunedin , New Zealand ; Department of Biochemistry, Otago School of Medical Sciences, University of Otago , Dunedin , New Zealand.
Front Oncol. 2013 Aug 30;3:225. doi: 10.3389/fonc.2013.00225. eCollection 2013.
GLI pathogenesis-related 1 (GLIPR1) was previously identified as an epigenetically regulated tumor suppressor in prostate cancer and, conversely, an oncoprotein in glioma. More recently, GLIPR1 was shown to be differentially expressed in other cancers including ovarian, acute myeloid leukemia, and Wilms' tumor. Here we investigated GLIPR1 expression in metastatic melanoma cell lines and tissue. GLIPR1 was variably expressed in metastatic melanoma cells, and transcript levels correlated with degree of GLIPR1 promoter methylation in vitro. Elevated GLIPR1 levels were correlated with increased invasive potential, and siRNA-mediated knockdown of GLIPR1 expression resulted in reduced cell migration and proliferation in vitro. Immunohistochemical studies of melanoma tissue microarrays showed moderate to high staining for GLIPR1 in 50% of specimens analyzed. GLIPR1 staining was observed in normal skin in merocrine sweat glands, sebaceous glands, and hair follicles within the dermis.
GLI 发病机制相关蛋白 1 (GLIPR1) 先前被鉴定为前列腺癌中受表观遗传调控的肿瘤抑制因子，而在神经胶质瘤中则是一种癌蛋白。最近，GLIPR1 在其他癌症中也表现出差异表达，包括卵巢癌、急性髓系白血病和肾母细胞瘤。在此，我们研究了 GLIPR1 在转移性黑素瘤细胞系和组织中的表达情况。GLIPR1 在转移性黑素瘤细胞中呈不同程度的表达，其转录水平与体外 GLIPR1 启动子甲基化程度相关。GLIPR1 水平升高与侵袭潜能增加相关，siRNA 介导的 GLIPR1 表达下调导致体外细胞迁移和增殖减少。对黑素瘤组织微阵列的免疫组织化学研究表明，分析的 50%标本中 GLIPR1 中度至高度染色。GLIPR1 染色见于真皮内的顶泌汗腺、皮脂腺和毛囊的正常皮肤中。
