Brujeni Gholamreza Nikbakht, Poorbazargani Taghi Taghi, Nadin-Davis Susan, Tolooie Mohammad, Barjesteh Neda
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
J Infect Dev Ctries. 2010 Oct 4;4(9):576-9. doi: 10.3855/jidc.711.
Bovine immunodeficiency virus (BIV) and bovine leukemia virus (BLV) have worldwide distributions, but their prevalences in Iran are unknown. We investigated the presence of infections in Iranian Holstein cattle and determined changes in hematological values for infected animals.
Nested PCR was used on blood samples from 143 animals Holstein cattle to detect proviral BIV and BLV gag sequences. Flow cytometric analysis was performed using monoclonal antibodies (mAbs) against CD4, CD8, and CD21 bovine T lymphocyte subsets.
Proviral BIV and BLV gag sequences were detected in 20.3% and 17% of the animals, respectively. BIV-BLV confection was also detected in 4.2% of the study population but this was not statistically significant. Flow cytometric analysis showed that both BIV-infected cows and non-infected ones had CD4/CD8 ratios of 2.45 and 1.43, respectively, and this difference was significant. BLV infected and non-infected animals had no significant differences in their CD4/CD8 ratio. In comparison to non-infected cattle, those with both BIV and BLV had a significant decrease in their CD4/CD8 ratios (1.5 % vs. 2.3; P = 0.01).
This is the first report of BIV and BLV infections in Iran. We found no evidence that infection with one agent predisposed an animal to infection with the other. BIV infection may have a role in decreasing T CD8 counts, but this may depend on the genetics of the cattle and virus strains involved.
牛免疫缺陷病毒（BIV）和牛白血病病毒（BLV）在全球范围内均有分布，但它们在伊朗的流行情况尚不清楚。我们调查了伊朗荷斯坦奶牛的感染情况，并确定了感染动物血液学值的变化。
采用巢式PCR对143头荷斯坦奶牛的血样进行检测，以检测前病毒BIV和BLV gag序列。使用针对牛T淋巴细胞亚群CD4、CD8和CD21的单克隆抗体（mAb）进行流式细胞术分析。
分别在20.3%和17%的动物中检测到前病毒BIV和BLV gag序列。在4.2%的研究群体中也检测到BIV-BLV混合感染，但这在统计学上不显著。流式细胞术分析表明，感染BIV的奶牛和未感染的奶牛的CD4/CD8比值分别为2.45和1.43，这种差异具有显著性。感染BLV和未感染的动物在CD4/CD8比值上没有显著差异。与未感染的牛相比，同时感染BIV和BLV的牛的CD4/CD8比值显著降低（1.5%对2.3；P = 0.01）。
这是伊朗关于BIV和BLV感染的首次报告。我们没有发现证据表明感染一种病原体使动物更容易感染另一种病原体。BIV感染可能在降低CD8 T细胞计数方面起作用，但这可能取决于所涉及的牛的遗传学和病毒株。
