Mishra Baijayantimala, Ragini S R, Kashiv I L, Ratho R K
Department of Virology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Indian J Pathol Microbiol. 2010 Oct-Dec;53(4):742-4. doi: 10.4103/0377-4929.72068.
Cell culture is the most popular method of virus propagation because of its high sensitivity. However, the need of high cost liquid nitrogen for storage of cell lines is one of the main limiting factor for its widespread use in developing countries.
The present study was therefore carried out to standardize the preservation of continuous cell lines at deep freezer (-85ºC) for 6 months.
Fixed number of Vero and Hep2 cells were preserved at -85ºC deep freezer in separate vials and were revived at one month interval to check the growth pattern.
Both the cell lines could be revived with healthy cells and monolayer was formed within 7-10 days, after storage at -85ºC for 4 months.
The present study highlights the utility of -85ºC deep freezer as an alternative to liquid N 2 for preservation of these cell lines at least up to four months.
由于细胞培养具有高灵敏度，它是最常用的病毒繁殖方法。然而，储存细胞系需要高成本的液氮，这是其在发展中国家广泛应用的主要限制因素之一。
因此，本研究旨在规范在深低温冰箱（-85ºC）中保存连续细胞系6个月的方法。
将固定数量的Vero细胞和Hep2细胞分别保存在-85ºC深低温冰箱的小瓶中，每隔一个月复苏一次以检查生长模式。
在-85ºC保存4个月后，两种细胞系均能复苏出健康细胞，并在7-10天内形成单层。
本研究强调了-85ºC深低温冰箱可作为液氮的替代品，用于保存这些细胞系至少4个月。
