用载胆固醇的普鲁兰纳米凝胶进行 DNA 分离。
DNA separation by cholesterol-bearing pullulan nanogels.
出版信息
Biomicrofluidics. 2010 Sep 30;4(3):32210. doi: 10.1063/1.3479997.
Abstract
We present an application of a novel DNA separation matrix, cholesterol-bearing pullulan (CHP) nanogels, for microchip electrophoresis. The solution of the CHP showed a unique phase transition around 30 mg∕ml and formed gel phase over this critical concentration. This gel phase consists of the weak hydrophobic interactions between the cholesterols could be easily deformed by external forces, and thus, loading process of the CHP nanogels into microchannels became easier. The high concentration of the CHP nanogels provided excellent resolutions especially for small DNA fragments from 100 to 1500 bp. The separation mechanism was discussed based on Ogston and Reptation models which had developed in gels or polymer solutions. The result of a single molecule imaging gave us an insight of the separation mechanism and the nanogel structures as well.
摘要
我们提出了一种新型 DNA 分离基质——载胆固醇支链淀粉纳米凝胶（CHP）在微芯片电泳中的应用。CHP 的溶液在 30mg/ml 左右表现出独特的相转变，并在这个临界浓度以上形成凝胶相。这种凝胶相由胆固醇之间的弱疏水相互作用组成，可以很容易地被外力变形，因此，CHP 纳米凝胶载入微通道的过程变得更加容易。CHP 纳米凝胶的高浓度特别为 100 至 1500bp 的小 DNA 片段提供了优异的分辨率。分离机制是基于在凝胶或聚合物溶液中发展起来的 Ogston 和 Reptation 模型进行讨论的。单分子成像的结果使我们深入了解了分离机制和纳米凝胶结构。
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2
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Lab Chip. 2006 Apr;6(4):526-33. doi: 10.1039/b515557f. Epub 2006 Mar 2.
3
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4
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