College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
Curr Microbiol. 2011 Mar;62(3):871-5. doi: 10.1007/s00284-010-9794-z. Epub 2010 Nov 4.
This article focuses on the relation between laccase-secreting ability and growing cycle in the edible fungi. First, laccase activities of fifteen different edible fungi were detected and determined by plate assay and spectrophotometric method using 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) as the substrate. The results showed the laccase-secreting ability in the edible fungi and their growing cycles are closely related. The edible fungi strains with short growing cycles originate from their high levels of secreted laccase activity. However, those strains require long growing cycles due to the low levels of secreted laccase, even no detectable laccase activity. The research provides the first evidence on the corresponding relation between the level of secreted laccase activity and growth cycles of edible fungi. Our study has significantly increased the understanding of the role of laccase in the growth and development of edible fungi.
本文主要关注产漆酶能力与可食用真菌生长周期之间的关系。首先,通过平板法和分光光度法,使用 2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)作为底物,检测并确定了 15 种不同可食用真菌的漆酶活性。结果表明,可食用真菌的产漆酶能力与其生长周期密切相关。生长周期短的可食用真菌菌株,其漆酶分泌活性水平较高。然而,由于漆酶分泌水平较低,甚至没有可检测到的漆酶活性,这些菌株的生长周期较长。本研究首次提供了可食用真菌漆酶分泌活性水平与其生长周期之间的对应关系的证据。我们的研究显著提高了对漆酶在可食用真菌生长发育过程中作用的认识。