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合成培养基和天然培养基对白腐真菌漆酶产生的影响。

Effect of synthetic and natural culture media on laccase production by white rot fungi.

作者信息

Kahraman Sibel S, Gurdal Ismail H

机构信息

Department of Science, Faculty of Education, Inonu University, Malatya, Turkey.

出版信息

Bioresour Technol. 2002 May;82(3):215-7. doi: 10.1016/s0960-8524(01)00193-6.

Abstract

Laccase is among the major enzymes of white rot fungi involved in lignocellulose degradation. The present paper reports its production by two white rot fungi (Coriolus versicolor, Funalia trogii) under different nutritional conditions. Various synthetic culture media and natural culture medium (molasses wastewater) were tested. Enzyme production in various synthetic culture media, molasses wastewater (vinasse) culture medium and in the absence or presence of cotton stalk supplements showed that vinasse culture medium was a better laccase-inducer medium than the synthetic culture medium. Addition of cotton stalk to various media enhanced the enzyme production. The highest laccase activity was obtained in vinasse culture medium with cotton stalk.

摘要

漆酶是参与木质纤维素降解的白腐真菌的主要酶类之一。本文报道了两种白腐真菌(云芝、毛栓菌)在不同营养条件下漆酶的产生情况。测试了各种合成培养基和天然培养基(糖蜜废水)。在各种合成培养基、糖蜜废水(酒糟)培养基以及有无棉秆添加物的情况下的酶产量表明,酒糟培养基比合成培养基更适合作为漆酶诱导培养基。向各种培养基中添加棉秆可提高酶产量。在添加棉秆的酒糟培养基中获得了最高的漆酶活性。

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