Chen Liding, Yan Miao, Qian Xin, Yang Ziwei, Xu Yanfei, Wang Tianjiao, Cao Jixuan, Sun Shujing
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
Front Microbiol. 2022 Jan 31;13:787628. doi: 10.3389/fmicb.2022.787628. eCollection 2022.
The effects of biological factors on the vegetative growth process of mushrooms remain largely unexplored. We investigated the bacterial community in different growth stages of by high-throughput sequencing technology to explore the relationship between interacting bacteria and the growth and development of We found significant variances in mushroom interacting association bacteria (MIAB) compositions among the samples from different growth stages, and 410 genera were identified. The bacteria in the full-bag and post-ripe stages were shifted to the biocontrol and growth-promotion ones. The mushroom growth-promoting bacteria (MGPB) were also isolated successfully and identified as Bac1. The growth speed and density of mycelial pellets of , and activities of two exoenzymes (laccase and amylase), were analyzed by adding the different volumes of cell-free fermentation broth of Bac1 to fungal culture media. The results showed that when a 5 mL cell-free fermentation broth was used, the growth speed of hyphae was enhanced by 1.15-fold over the control and reached 0.46 mm/h. The relative activity of laccase and amylase was increased by 26.9 and 43.83%. Our study revealed that the abundant interacting bacteria coexist with hyphae. Moreover, the abundance of some bacteria exhibiting a positive correlation with the growth periods of their host fungi can effectively promote the growth of the host, which will provide technical supports on the high-efficiency production of in factory cultivation.
生物因素对蘑菇营养生长过程的影响在很大程度上仍未得到探索。我们通过高通量测序技术研究了蘑菇不同生长阶段的细菌群落，以探讨相互作用的细菌与蘑菇生长发育之间的关系。我们发现不同生长阶段样本中蘑菇相互作用关联细菌（MIAB）的组成存在显著差异，共鉴定出410个属。满袋期和后熟期的细菌转变为具有生物防治和促生长作用的细菌。我们还成功分离出了蘑菇促生长细菌（MGPB）并鉴定为芽孢杆菌Bac1。通过向真菌培养基中添加不同体积的芽孢杆菌Bac1无细胞发酵液，分析了蘑菇菌丝球的生长速度和密度以及两种胞外酶（漆酶和淀粉酶）的活性。结果表明，当使用5 mL无细胞发酵液时，蘑菇菌丝的生长速度比对照提高了1.15倍，达到0.46 mm/h。漆酶和淀粉酶的相对活性分别提高了26.9%和43.83%。我们的研究表明，丰富的相互作用细菌与蘑菇菌丝共存。此外，一些与宿主真菌生长时期呈正相关的细菌丰度能够有效促进宿主生长，这将为工厂化栽培中蘑菇的高效生产提供技术支持。
