[高效液相色谱法测定决明子中橙黄决明素和大黄酚的含量]
[Determination of aurantio-obtusin and chrysophanol in cassiae semen by HPLC].
作者信息
Huang Xiaoping, Zhong Guoyue, Zhang Xuemei, Zhang Yi
机构信息
Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2065-7.
OBJECTIVE
To improve the determination method of Cassiae Semen in China Pharmacopeia edition 2005. MMETHOD: Aurantio-obtusin and chrysophanol were separated on an Inertsil ODS-3 column with acetonitrile-0.1% phosphoric acid solutiona as mobile phase in gradient elution.
RESULT
The linear ranges of aurantio-obtusin and chrysophanol were 2.3-230, 2.87-286 mg x L(-1), respectively. The average recover were 100.3% (RSD 3.1%), and 99.5% (RSD 2.5%) respectively.
CONCLUSION
This method is specific, accurate and reproducible, and can be used for the assay of Cassiae Semen in the new edition of Chinese pharmacopoeia.
目的
改进《中国药典》2005年版中决明子的测定方法。方法：采用Inertsil ODS - 3柱，以乙腈 - 0.1%磷酸溶液为流动相进行梯度洗脱，分离橙黄决明素和大黄酚。结果：橙黄决明素和大黄酚的线性范围分别为2.3 - 230、2.87 - 286 mg·L⁻¹。平均回收率分别为100.3%（RSD 3.1%）和99.5%（RSD 2.5%）。结论：该方法专属性强、准确、重现性好，可用于新版《中国药典》中决明子的含量测定。
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