[Chromatographic fingerprint peak matching of Semen Cassiae by asymptotic window orthoganality projection analytical method].

OBJECTIVE

To investigate the principle and basis about the chromatographic fingerprint peak matching of Semen Cassiae by the asymptotic window orthogonal projection analytical method.

METHOD

The samples of Semen Cassiae were hydrolyzed in the 1.5 mol x L(-1) hydrochloride acid and then reflux extracted with chloroform. The chromatographic condition was that the HPLC was run on Agilent 1100 column, Zorbax Eclipse XDB-C18 (4.6 mm x 250 mm, 5 microm). The protected column was Scienhome C18 column (3.0 mm x 20 mm, 5 microm) with acetonitrile-water (0.1% phosphoric acid) as mobile phase in gradient elution at a flow rate of 1.0 mL x min(-1). The detection wavelength and reference wavelength were 278 nm and 550 nm, respectively.

RESULT

The characteristic fingerprint collection of illustrative plates of Semen Cassiae were obtained with different HPLC instruments and chromatographic columns. And the asymptotic window orthogonal projection analytical method was used to decide the chromatographic fingerprint peak matching of Semen Cassiae, promptly and prapidly.

CONCLUSION

The asymptotic window orthogonal projection analytical method can progress the matching of different fingerprint collection of illustrative plates peak matching well and truly.