Chen Yuping, Bi Dan, Tu Pengfei
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2068-71.
To establish the quality standard of Sappan Lignum, TLC and HPLC method were employed. The components of Sappan Lignum could be separated well on GF254 thin layer plate using a mixture of chloroform-acetone-formic acid (8:4:1) as the mobile phase, and the 18 samples collected basically showed the same spots. The ethanol-soluble extractives of 18 samples varied in the range of 6.4% to 11.3%. The methodological investigation of assay showed, the peak areas and the injection amount of Brazilin and (+/-) protosappanin B were in good linear correlation when their amounts were in the ranges of 0.362-5.425 microg and 0.313-4.695 microg, with the regression equations of Y = 798,999.57X - 219,666.54 (r = 0.9997) and Y = 930,296.63X - 123,330.67 (r = 0.9995) and the average recoveries were 98.6% and 100.5%, respectively. The contents differed significantly among the samples. The TLC identification method established was suitable to identify Sappan Lignum due to its strong specificity. The HPLC assay method established could be applied to the quality control of Sappan Lignum due to its convenience, good reproducibility and high accuracy.
为建立苏木的质量标准，采用了薄层色谱法（TLC）和高效液相色谱法（HPLC）。以氯仿 - 丙酮 - 甲酸（8:4:1）混合液为流动相，苏木的成分在GF254薄层板上能得到良好分离，所采集的18个样品基本呈现相同斑点。18个样品的乙醇提取物含量在6.4%至11.3%之间。含量测定方法学考察表明，巴西苏木素和（±）原苏木素B进样量在0.362 - 5.425μg和0.313 - 4.695μg范围内时，峰面积与进样量呈良好线性关系，回归方程分别为Y = 798,999.57X - 219,666.54（r = 0.9997）和Y = 930,296.63X - 123,330.67（r = 0.9995），平均回收率分别为98.6%和100.5%。不同样品间含量差异显著。所建立的TLC鉴别方法特异性强，适用于苏木的鉴别。所建立的HPLC含量测定方法操作简便、重现性好、准确度高，可用于苏木的质量控制。
