Meng He, Chen Yuping, Qin Wenjie, Tang Xiaojing, Ye Zuguang
National Engineering Research Center for Research and Development of Traditional Chinese Medicine Multi-ingredient Drugs, Beijing 100075, China.
Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2072-4.
To establish a HPLC method for the determination of vaccarin in Vaccariae Semen. Analysis was carried out on an Alltima-C18 column (4.6 mm x 250 mm, 5 microm) eluted with methanol -0.3% phosphoric acid as mobile phase in gradient elution. The flow rate was 1.0 mL x min(-1) and detected wavelength was set at 280 nm. The peak areas and injection ammounts of vaccarin showed a good linear relationship in the range of 0.102-1.539 microg, R2 = 0.9997. The average recovery was 100.4%, RSD was 0.81%. The results of the assay of 10 samples showed that the contents of vaccarin varied in the range of 0.46%-0.57%. The method is simple, accurate, reproducible and specific. It can be used for the quality control of Vaccariae Semen.
