Analysis and Testing Centre, Dezhou University, Dezhou, P. R. China; Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan, P. R. China; Key Laboratory of Coordination Chemistry and Functional Materials in Universities of Shandong, Dezhou University, Dezhou, P. R. China.
Electrophoresis. 2013 Dec;34(24):3326-32. doi: 10.1002/elps.201300402.
A method was developed to determine brazilin and protosappanin B in natural products by CE after acid barrage stacking. The optimum conditions were as follows: a BGE of 20 mM sodium tetraborate (pH 9.2) containing 6% v/v of methanol, hydrodynamic injection (0.5 psi, 65 s) followed by hydrodynamic injection of 150 mM citric acid (pH 2.3; 0.5 psi, 22 s), and separated with +25 kV. Under these conditions, brazilin and protosappanin B were separated with a sample-to-sample time less than 13 min and detection limits of 0.28 μg/mL and 0.15 μg/mL, respectively. The applicability of the developed method was demonstrated by the detection of brazilin and protosappanin in methanol extract of sappan lignum.
建立了一种通过酸堆积 CE 测定天然产物中巴豆油素和原巴西苏木素 B 的方法。最佳条件为：BGE 为 20 mM 四硼酸钠（pH 9.2），含 6% v/v 甲醇，采用液压进样（0.5 psi，65 s），随后进样 150 mM 柠檬酸（pH 2.3；0.5 psi，22 s），分离电压为+25 kV。在此条件下，巴豆油素和原巴西苏木素 B 的分离时间不到 13 min，检测限分别为 0.28 μg/mL 和 0.15 μg/mL。该方法已应用于甲醇提取苏木中的巴豆油素和原巴西苏木素 B 的检测。
