高效液相色谱-蒸发光散射检测法测定通关藤中通关藤苷H

[Determination of tenacissoside H in Marsdeniae tenacissimae by HPLC-ELSD].

作者信息

Li Huali, Lu Yan, Hu Jiayun, Chen Daofeng

机构信息

Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2083-6.

PMID:21046734

Abstract

In order to improve the quality standard of Marsdenia tenacissima, a quantitative determination method of tenacissoside H was developed using high performance liquid chromatography. The method was carried out on a YMC ODS-H80 (4.6 mm x 250 mm, 4 microm) column eluted with a mixture of acetonitrile and water (50:50) as the mobile phase. The flow rate was 0.8 mL x min(-1) and the column temperature was 35 degrees C. An evaporative light scattering detector (ELSD) was used with the temperature of drift tube set at 60 degrees C and the gas flow rate of nitrogen set at 1.5 mL x min(-1). The calibration curve was linear in the range from 0.5625 to 36.00 microg (r = 0.9998). The average recovery and RSD were 99.41% and 1.8%, respectively. The contents of tenacissoside H in the 11 samples from different habitats varied from 0.201% to 0.862%. The method established in this paper is specific and reliable to control and evaluate the quality of M. tenacissima.

摘要

为提高通关藤的质量标准，建立了一种采用高效液相色谱法测定通关藤苷H含量的方法。该方法采用YMC ODS - H80（4.6 mm×250 mm，4μm）色谱柱，以乙腈和水（50:50）的混合溶液作为流动相进行洗脱。流速为0.8 mL·min⁻¹，柱温为35℃。采用蒸发光散射检测器（ELSD），漂移管温度设定为60℃，氮气气流速度设定为1.5 mL·min⁻¹。校准曲线在0.5625至36.00μg范围内呈线性（r = 0.9998）。平均回收率和相对标准偏差分别为99.41%和1.8%。来自不同产地的11个样品中通关藤苷H的含量在0.201%至0.862%之间。本文建立的方法对于控制和评价通关藤的质量具有特异性和可靠性。