高效液相色谱-蒸发光散射检测法测定藜芦中藜芦胺的含量
[Determination of vetatramine in Veratrum nigrum by HPLC-ELSD].
作者信息
Wang Li-Shu, Zhao Da-Qing, Tao Hua-Ming, Liu Yong-Hong
机构信息
College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Apr;33(7):791-2.
OBJECTIVE
To develop an HPLC-ELSD method for determination of vetatramine. in Veratrum nigrum.
METHOD
The analy fical column was Shim-pack ODS - C18 (4.6 mm x 250 mm, 4 microm) column, the mobile phase was acetonitrile-water (containing 0.1% triethylamine) (50:50), at a flow rate of 0.8 mL x min(-1). The temperature of drift tube was 90 degrees C and the gas flow was at the rate of 2.5 L x min(-1).
RESULT
The calibration curve was linear in the range of 0.36-3.6 microg (r = 0.999 8). The average recovery was 100.9% (RSD 2.3%, n = 6). The contents of veratramine in Veratrum nigrum. from the ten different sources were determined.
CONCLUSION
The method may be used as a accurate and reproducible way to determine the content of veratramine in V. nigrum.
目的
建立一种用于测定藜芦中藜芦胺含量的高效液相色谱-蒸发光散射检测法。
方法
分析柱为岛津Shim-pack ODS - C18（4.6 mm×250 mm，4 µm）柱，流动相为乙腈-水（含0.1%三乙胺）（50:50），流速为0.8 mL·min⁻¹。漂移管温度为90℃，气体流速为2.5 L·min⁻¹。
结果
校准曲线在0.36 - 3.6 µg范围内呈线性（r = 0.999 8）。平均回收率为100.9%（RSD 2.3%，n = 6）。测定了来自十个不同来源的藜芦中藜芦胺的含量。
结论
该方法可作为准确、可重现地测定藜芦中藜芦胺含量的方法。
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