Lee Hee Uk, Shin Hyun Yong, Lee Jin Young, Song Yoon Seok, Park ChulHwan, Kim Seung Wook
Department of Chemical and Biological Engineering, Korea University, 5 Ga, Anam-Dong, Sungbuk-Gu, Seoul 136-701, Korea.
J Agric Food Chem. 2010 Dec 8;58(23):12096-100. doi: 10.1021/jf102784t. Epub 2010 Nov 3.
A sandwich-type immunosensor composed of antigen-double target/probe DNA-coated gold nanoparticles (NPs) was developed for the measurement of fluorescence intensity and quantitative analysis of single-stranded DNA based on the concentration of free glyphosate. The reaction between the antigen-double DNA-gold NPs and immobilized antibody on the substrate was carried out for 2 h. The results of the antigen-antibody reaction were measured on the basis of the fluorescence intensity obtained from comparison with the free antigens at concentrations of 0.01-100 μg mL(-1) for the detection of immobilized antigen-double DNA-gold NPs. For the quantitative analysis based on the concentration of glyphosate(0.01-100 μg mL(-1)), the immunosensor response also revealed the same detection range of glyphosate using DNA detection.
