Wang Duo, Lin Bixia, Cao Yujuan, Guo Manli, Yu Ying
School of Chemistry and Environment, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou , Guangzhou, Guangdong 510006, China.
J Agric Food Chem. 2016 Aug 3;64(30):6042-50. doi: 10.1021/acs.jafc.6b01088. Epub 2016 Jul 22.
A sensitive fluorescence detection method for glyphosate (GLY) was established based on immune reaction. First, carbon dot labeled antibodies (lgG-CDs) which were able to specifically identify glyphosate were prepared with the environmentally friendly carbon dots (CDs) and glyphosate antibody (lgG). lgG-CDs could be used to in situ visualize the distribution of glyphosate in plant tissues. In order to eliminate the effects of excess lgG-CDs on the determination of GLY, antigen magnetic beads Fe3O4-GLY based on magnetic nanoparticles Fe3O4 and glyphosate were constructed and utilized to couple with the excess lgG-CDs. After magnetic separation to remove antigen magnetic beads, there was a linear relationship between the fluorescence intensity of lgG-CDs and the logarithmic concentration of glyphosate in the range of 0.01-80 μg/mL with a detection limit of 8 ng/mL. The method was used for the detection of glyphosate in Pearl River water, tea, and soil samples with satisfactory recovery ratio between 87.4% and 103.7%.
基于免疫反应建立了一种灵敏的草甘膦（GLY）荧光检测方法。首先，用环境友好型碳点（CDs）和草甘膦抗体（lgG）制备了能够特异性识别草甘膦的碳点标记抗体（lgG-CDs）。lgG-CDs可用于原位可视化草甘膦在植物组织中的分布。为消除过量lgG-CDs对GLY测定的影响，构建了基于磁性纳米颗粒Fe3O4和草甘膦的抗原磁珠Fe3O4-GLY，并用于与过量的lgG-CDs偶联。通过磁分离去除抗原磁珠后，lgG-CDs的荧光强度与草甘膦对数浓度在0.01-80μg/mL范围内呈线性关系，检测限为8ng/mL。该方法用于珠江水、茶叶和土壤样品中草甘膦的检测，回收率在87.4%至103.7%之间，结果令人满意。
