The School of Pre-clinical Sciences, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, China.
Clin Biochem. 2011 Mar;44(4):331-6. doi: 10.1016/j.clinbiochem.2010.10.008. Epub 2010 Nov 1.
To detect serum antibody against SMP30 in HCC patients and to evaluate its potential associations with HCC patient's clinical parameter and expression levels in HCC tissues.
Serum antibody to SMP30 was tested by ELISA method; SMP30 mRNA and protein expression in HCC patients were analyzed using the methods of in situ nucleic acid hybridization and immunohistochemistry, respectively.
The highest relevance of SMP30 antibody was associated with HCC (32.4%). The positive rate of SMP30 antibody was not related to the age of patients, tumor size, metastasis and infections of HBV, but the positive rate for SMP30 antibody in the HCC sera with alpha-fetoprotein (AFP) negative was higher (43.6%) compared with that AFP positive (26.2%). Both SMP30 mRNA and protein expression levels were downregulated in HCC and upregulated in adjacent tissues.
SMP30 may be useful for HCC serologic screening, especially for the patients with AFP negative.
检测 HCC 患者血清中 SMP30 抗体，并评估其与 HCC 患者临床参数及 HCC 组织中表达水平的潜在相关性。
采用 ELISA 法检测 SMP30 血清抗体；采用原位核酸杂交和免疫组织化学方法分别分析 HCC 患者中 SMP30 mRNA 和蛋白的表达。
SMP30 抗体与 HCC 的相关性最高（32.4%）。SMP30 抗体的阳性率与患者年龄、肿瘤大小、转移和 HBV 感染无关，但 AFP 阴性 HCC 血清中 SMP30 抗体的阳性率（43.6%）高于 AFP 阳性（26.2%）。SMP30 mRNA 和蛋白在 HCC 中表达下调，在相邻组织中表达上调。
SMP30 可能对 HCC 的血清学筛查有用，特别是对 AFP 阴性的患者。
