Free University Berlin, Institute for Chemistry and Biochemistry, Berlin, Germany.
Biomaterials. 2011 Feb;32(5):1311-6. doi: 10.1016/j.biomaterials.2010.10.010. Epub 2010 Nov 2.
We report the preparation of polyglycerol particles on different length scales by extending the size of hyperbranched polyglycerols (3 nm) to nanogels (32 nm) and microgels (140 and 220 μm). We use miniemulsion templating for the preparation of nanogels and microfluidic templating for the preparation of microgels, which we obtain through a free-radical polymerization of hyperbranched polyglycerol decaacrylate and polyethylene glycol-diacrylate. The use of mild polymerization conditions allows yeast cells to be encapsulated into the resultant microgels with cell viabilities of approximately 30%.
我们通过将超支化聚甘油（3nm）的尺寸扩展到纳米凝胶（32nm）和微凝胶（140 和 220μm），制备了不同长度尺度的聚甘油颗粒。我们使用亚微米乳液模板法制备纳米凝胶和微流控模板法制备微凝胶，通过超支化聚甘油癸烯酸酯和聚乙二醇二丙烯酸酯的自由基聚合得到它们。温和的聚合条件允许将酵母细胞包封在所得微凝胶中，细胞活力约为 30%。
