Liu Dongyou, Lawrence Mark L, Gorski Lisa, Mandrell Robert E, Ainsworth A Jerald, Austin Frank W
College of Veterinary Medicine, Mississippi State University, MS 39762, USA.
J Clin Microbiol. 2006 Jan;44(1):214-7. doi: 10.1128/JCM.44.1.214-217.2006.
A collection of Listeria monocytogenes serotype 4b strains belonging to lineages I and III were examined by PCR and Southern blot analysis using species-, virulence-, and serotype-specific primers and probes. Whereas four serotype 4b lineage I strains reacted in PCR with the serotype 4b-, 4d-, and 4e-specific ORF2110 and virulence-specific lmo1134 and lmo2821 primers, all nine serotype 4b lineage III strains were negative by ORF2110 and lmo1134 primers. In addition, the nine serotype 4b lineage III strains formed two separate groups through their reactions in PCR with virulence-specific lmo2821 primers. Southern blot analysis using species-specific lmo0733 and virulence-specific lmo2821 gene probes largely confirmed the PCR results. These findings indicate that L. monocytogenes serotype 4b strains belonging to lineages I and III possess distinct molecular features.
使用种属特异性、毒力特异性和血清型特异性引物及探针，通过PCR和Southern印迹分析，对属于谱系I和III的一组单核细胞增生李斯特菌血清型4b菌株进行了检测。虽然4株血清型4b谱系I菌株在PCR中与血清型4b、4d和4e特异性的ORF2110以及毒力特异性的lmo1134和lmo2821引物发生反应，但所有9株血清型4b谱系III菌株对ORF2110和lmo1134引物均呈阴性。此外，这9株血清型4b谱系III菌株在与毒力特异性lmo2821引物的PCR反应中形成了两个不同的组。使用种属特异性lmo0733和毒力特异性lmo2821基因探针的Southern印迹分析在很大程度上证实了PCR结果。这些发现表明，属于谱系I和III的单核细胞增生李斯特菌血清型4b菌株具有不同的分子特征。
