Selmaj K W, Farooq M, Norton W T, Raine C S, Brosnan C F
Department of Pathology (Neuropathology), Albert Einstein College of Medicine, Bronx, NY 10461.
J Immunol. 1990 Jan 1;144(1):129-35.
The effect of cytokines on astrocytes cultured from mature bovine brain was determined both in a serum-containing medium and in a chemically-defined medium. The results showed that in serum-free medium, human TNF and, to a lesser degree, IL-6 and lymphotoxin, were mitogenic for astrocytes. Increased uptake of [3H]thymidine could be detected within 36 h in vitro and its presence in astrocytes was confirmed by autoradiography. In contrast, neither IL-1 alpha nor IL-1 beta induced astrocyte proliferation in serum-free medium but showed some synergistic effect with serum after 72 h. The proliferative effect of TNF and IL-6 was confirmed by cell counting. None of the cytokines tested was toxic for astrocytes as measured by 51Cr release. No mitogenic effect for oligodendroglia, purified from the same source, was detected. The results support a role for products of activated inflammatory cells in the development of astrocyte proliferation that may contribute to the reactive gliosis found in white matter diseases of the central nervous system such as multiple sclerosis.
在含血清培养基和化学成分明确的培养基中，研究了细胞因子对从成年牛脑培养的星形胶质细胞的影响。结果表明，在无血清培养基中，人肿瘤坏死因子（TNF）以及程度较轻的白细胞介素-6（IL-6）和淋巴毒素对星形胶质细胞具有促有丝分裂作用。体外36小时内可检测到[3H]胸腺嘧啶核苷摄取增加，放射自显影证实其存在于星形胶质细胞中。相比之下，白细胞介素-1α（IL-1α）和白细胞介素-1β（IL-1β）在无血清培养基中均未诱导星形胶质细胞增殖，但72小时后与血清显示出一定的协同作用。通过细胞计数证实了TNF和IL-6的增殖作用。通过51Cr释放测定，所测试的细胞因子对星形胶质细胞均无毒性。未检测到从同一来源纯化的少突胶质细胞有促有丝分裂作用。这些结果支持活化炎症细胞产物在星形胶质细胞增殖发展中起作用，这可能有助于中枢神经系统白质疾病如多发性硬化症中发现的反应性胶质增生。
